![Western Blot: Cyclin D2 Antibody [NBP3-13225] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D2 antibody (NBP3-13225) diluted at 1:1000.](http://images.novusbio.com/fullsize/Cyclin-D2-Antibody-Western-Blot-NBP3-13225-img0001.jpg "Western Blot: Cyclin D2 Antibody [NBP3-13225] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D2 antibody (NBP3-13225) diluted at 1:1000.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Cyclin D2. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CCND2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 33 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cyclin D2 Antibody (NBP3-13225)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CCND2 |
![Immunocytochemistry/Immunofluorescence: Cyclin D2 Antibody [NBP3-13225] - Cyclin D2 antibody detects Cyclin D2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin D2 protein stained by Cyclin D2 antibody (NBP3-13225) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Cyclin-D2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13225-img0003.jpg "Immunocytochemistry/Immunofluorescence: Cyclin D2 Antibody [NBP3-13225] - Cyclin D2 antibody detects Cyclin D2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin D2 protein stained by Cyclin D2 antibody (NBP3-13225) diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: Cyclin D2 Antibody [NBP3-13225] - Cyclin D2 antibody detects Cyclin D2 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. Cyclin D2 antibody (NBP3-13225) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Cyclin-D2-Antibody-Immunohistochemistry-Paraffin-NBP3-13225-img0002.jpg "Immunohistochemistry-Paraffin: Cyclin D2 Antibody [NBP3-13225] - Cyclin D2 antibody detects Cyclin D2 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. Cyclin D2 antibody (NBP3-13225) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")