Cyclin D1/D2 Antibody [Unconjugated] - Cross-reactive

Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, U2OS human osteosarcoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse Cyclin D1/D2 Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4196) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human Cyclin D1, D2, and D3 (5 ng/lane) were included. A specific band for Cyclin D1 was detected at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MCF‑7 human breast cancer cell line was stained with Goat Anti-Human/Mouse Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4196, filled histogram) or isotype control AA175antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldeyde and permeabilized with saponin.

• Reactivity: Hu, Mu
• Applications: WB, CyTOF-ready, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Cyclin D1/D2 Antibody [Unconjugated] - Cross-reactive Summary

• Immunogen: E. coli-derived recombinant human Cyclin D1
  Met1-Ile295
  Accession # P24385
• Specificity: Detects human and mouse Cyclin D1/D2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCND1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cyclin D1/D2 Antibody [Unconjugated] - Cross-reactive

• BCL1;CCND1;D11S287E;G1/S-specific cyclin-D1;PRAD1;U21B31
• Cyclin D1/D2

Background

The D-type cyclins (cyclins D1, D2, and D3) and their associated kinases, Cdk4 and Cdk6, play an important role in the progression from G₀/G₁ to S phase in the mammalian cell cycle. Cyclin D complexes containing Cdk4 or Cdk6 phosphorylate the retinoblastoma protein (pRb). pRb phosphorylation leads to the release of E2F transcription factors, which activate the expression of S-phase genes and thereby induce cell cycle progression. Cyclin D1, independent of Cdk4 activity, functions as a transcriptional modulator by regulating the activity of several transcription factors, such as estrogen receptor, Myb, and STAT3. Cyclin D1 has also been linked to the development and progression of several cancers including breast, bladder, esophagus, and lung. Overexpression of Cyclin D2 has been reported in gastric carcinoma, ovarian granulose cell tumors, and hematopoietic cell cancers, while in breast cancers, cyclin D2 expression is undetectable in 80% of the tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cyclin D1/D2 Antibody (AF4196)(11)

Publications using AF4196

• Wolf, B;Muralidharan, P;Lee, MY;Hua, W;Green, E;Wang, H;Strange, C; Overexpression of Alpha-1 Antitrypsin Increases the Proliferation of Mesenchymal Stem Cells by Upregulation of Cyclin D1 International journal of molecular sciences 2024-02-07 [PMID: 38396691] (Western Blot, ICC, Human)
• Wolf, B;Muralidharan, P;Lee, M;Hua, W;Green, E;Wang, H;Strange, C; Overexpression of Alpha-1 Antitrypsin Increases the Proliferation of Mesenchymal Stem Cells by Upregulation of Cyclin D1 and is Independent of the Wnt Signaling Pathway bioRxiv : the preprint server for biology 2023-10-28 [PMID: 37961658] (Western Blot, Immunocytochemistry/ Immunofluorescence, Human)
• Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J; Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers Biosensors 2023-08-27 [PMID: 37754086] (IHC, Mouse)
• XF Liu, K Wang, HC Du LncRNA SNHG6 regulating Hedgehog signaling pathway and affecting the biological function of gallbladder carcinoma cells through targeting miR-26b-5p Eur Rev Med Pharmacol Sci, 2020-07-01;24(14):7598-7611. 2020-07-01 [PMID: 32744686] (Western Blot, Human)
• Peng Zhang, Long Zhou, Lei Wang, Qirong Dong A Novel Nanofiber Hydrogel Loaded with Platelet-Rich Plasma Promotes Wound Healing Through Enhancing the Survival of Fibroblasts Medical Science Monitor 11/18/2019 [PMID: 31739310]
• Guangye Han, Xinjun Zhang, Pei Liu, Quanfeng Yu, Zeyu Li, Qinnan Yu et al. Knockdown of anti-silencing function 1B histone chaperone induces cell apoptosis via repressing PI3K/Akt pathway in prostate cancer International Journal of Oncology 8/16/2018 [PMID: 30132513]
• YJ Liu, W Li, F Chang, JN Liu, JX Lin, DX Chen MicroRNA-505 is downregulated in human osteosarcoma and regulates cell proliferation, migration and invasion Oncol. Rep., 2017-12-11;39(2):491-500. 2017-12-11 [PMID: 29251324] (Western Blot, Human)
• Muruganandan S, Govindarajan R, McMullen N, Sinal C Chemokine-Like Receptor 1 Is a Novel Wnt Target Gene that Regulates Mesenchymal Stem Cell Differentiation. Stem Cells, 2016-11-11;35(3):711-724. 2016-11-11 [PMID: 27733019] (Western Blot, Mouse)
• Q Chen, S Qin, Y Liu, M Hong, CN Qian, ET Keller, J Zhang, Y Lu IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker Oncotarget, 2016-10-18;7(42):68140-68150. 2016-10-18 [PMID: 27623076] (Western Blot, Human)
• MacLeod R Extracellular calcium-sensing receptor/PTH knockout mice colons have increased Wnt/beta-catenin signaling, reduced non-canonical Wnt signaling, and increased susceptibility to azoxymethane-induced aberrant crypt foci. Lab Invest, 2013-04-01;93(5):520-7. 2013-04-01 [PMID: 23545937] (Western Blot, Mouse)
• Muruganandan S, Parlee SD, Rourke JL, Ernst MC, Goralski KB, Sinal CJ Chemerin, a novel peroxisome proliferator-activated receptor gamma (PPARgamma) target gene that promotes mesenchymal stem cell adipogenesis. J. Biol. Chem., 2011-05-14;286(27):23982-95. 2011-05-14 [PMID: 21572083] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CCND1
• Uniprot:
  • P24385()