CXCL15/Lungkine Antibody (96708)

• Reactivity: Mu
• Applications: WB, ELISA(Cap)
• Clone: 96708
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CXCL15/Lungkine Antibody (96708) Summary

• Immunogen: E. coli-derived recombinant mouse CXCL15/Lungkine
  Gln26-Ala167
  Accession # Q9WVL7
• Specificity: Detects mouse CXCL15/Lungkine in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse (rm) CXCL13, recombinant human (rh) CXCL13, rhCXCL5, rhCXCL8, rmMIP-2, recombinant rat CINC-2 beta , rmCX3CL1, rmCCL12, or rhCCL15 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: CXCL15
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Mouse CXCL15/Lungkine Biotinylated Antibody (Catalog number BAF442)
  Standard: Recombinant Mouse CXCL15/Lungkine (Catalog number 442-LK)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL15/Lungkine Antibody (96708)

• CXCL15
• Lungkine
• Scyb15
• Weche

Background

Mouse Lungkine/CXCL15 (1), also named WECHE (2), is a member of the ELR motif-containing CXC chemokines. The cDNA of mouse Lungkine encodes a protein of 166 amino acids (aa) with a 25 aa predicted signal peptide and a 141 aa mature protein with an extremely long C‑terminal tail that protrudes beyond the chemokine fold. Mouse Lungkine shares 35% aa sequence identity with human CXCL5 and 31% identity with human CXCL8. The gene for mouse Lungkine has been mapped to chromosome 5. By Northern blot and in situ hybridization, Lungkine transcripts are only specifically detected in the adult and fetal lung, and its expression is up‑regulated under inflammatory conditions (1). Lungkine protein is secreted into bronchoalveolar space and is involved in lung-specific neutrophils trafficking (1). Studies from Lungkine knock out mice suggests that Lungkine is an important mediator of neutrophil migration from the lung parenchyma into the airspace (3). Lungkine is also chemotactic for bone marrow progenitor cells and modulates hematopoietic cell differentiation (2).

1. Rossi, D.L. et al. (1999) J. Immunol. 162:5490.
2. Ohneda, O. et al. (2000) Immunity 12:141.
3. Chen, S-C. et al. (2001) J. Immunol. 166:3362.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL15/Lungkine Antibody (MAB442)(2)

Publications using MAB442

• Karisola P, Lehto M, Kinaret P, Ahonen N, Haapakoski R, Anthoni M, Taniguchi M, Wolff H, Puustinen A, Alenius H Invariant Natural Killer T Cells Play a Role in Chemotaxis, Complement Activation and Mucus Production in a Mouse Model of Airway Hyperreactivity and Inflammation. PLoS ONE, 2015-06-12;10(6):e0129446. 2015-06-12 [PMID: 26067998] (Western Blot, Mouse)
• Maheshwari A, Christensen RD, Calhoun DA Circulating CXC-chemokine concentrations in a murine intestinal ischemia-reperfusion model. Fetal Pediatr Pathol, 2004-03-01;23(2):145-57. 2004-03-01 [PMID: 15768860] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: CXCL15
• Uniprot:
  • Q9WVL7()