CUL4B Antibody

Western Blot: CUL4B Antibody [25150002] - Samples: Lane 1, Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight [kDa]: 104, 103, 102 (splice variants) Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.

Immunohistochemistry: CUL4B Antibody [25150002] - Most of the normal and malignant tissues showed moderate to strong nuclear staining with an additional weak cytoplasmic positivity. Normal liver was negative. Most hepatocellular carcinomas were weakly stained. Image and statement courtesy of the Human Protein Atlas (HPA).

ELISA: CUL4B Antibody [25150002]

Product Discontinued

CUL4B Antibody Summary

• Immunogen: This antibody is specific for the C Terminus Region of the target protein.
• Specificity: This product is specific for Human CUL4B.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CUL4B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:5000-1:20000
• Application Notes: This antibody is useful in ELISA, Immunohistochemistry-Paraffin and Western Blot.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for CUL4B Antibody

• CUL-4B
• cullin 4B
• cullin-4B
• DKFZp686F1470
• KIAA0695
• MRXHF2
• MRXS15
• MRXSC
• SFM2

Background

This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CUL4B Antibody (25150002)(2)

Publications using 25150002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: CUL4B
• Entrez:
  • 8450(Human)
• Uniprot:
  • Q13620(Human)