CTDSPL Overexpression Lysate (Native)

Images

 
Western Blot: CTDSPL Overexpression Lysate (Adult Normal) [NBL1-09569] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CTDSPL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CTDSPL Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC214440 and based on accession number NM_005808. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like (CTDSPL), transcript variant 2, mRNA.
Gene
CTDSPL

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CTDSPL

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CTDSPL Overexpression Lysate (Native)

  • C3orf8
  • Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3
  • CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) smallphosphatase-like
  • CTD small phosphatase-like protein
  • CTDSP-like
  • EC 3.1.3
  • HYA22
  • NIF1
  • NIFL
  • NIF-like protein
  • NLI-interacting factor 1
  • Nuclear LIM interactor-interacting factor 1
  • Protein YA22
  • PSR1
  • RB protein serine phosphatase from chromosome 3
  • RBSP3EC 3.1.3.16
  • SCP3chromosome 3 open reading frame 8
  • Small CTD phosphatase 3
  • Small C-terminal domain phosphatase 3
  • YA22

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol CTDSPL