CTDSPL Antibody

Western Blot: CTDSPL Antibody [NBP2-76897] - Western blot analysis of CTDSPL on HepG2 cell lysate using anti-CTDSPL antibody at 1/500 dilution.

Flow Cytometry: CTDSPL Antibody [NBP2-76897] - Flow cytometric analysis of HepG2 cells with CFTR antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Product Discontinued

CTDSPL Antibody Summary

• Immunogen: Recombinant protein within Human CTDSPL aa 77-252 / 276. (SwissProt: O15194 Human; SwissProt: P58465 Mouse; Unigene: 37030 Rat)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CTDSPL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Western Blot 1:500
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CTDSPL Antibody

• C3orf8
• Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3
• CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) smallphosphatase-like
• CTD small phosphatase-like protein
• CTDSP-like
• EC 3.1.3
• HYA22
• NIF1
• NIFL
• NIF-like protein
• NLI-interacting factor 1
• Nuclear LIM interactor-interacting factor 1
• Protein YA22
• PSR1
• RB protein serine phosphatase from chromosome 3
• RBSP3EC 3.1.3.16
• SCP3chromosome 3 open reading frame 8
• Small CTD phosphatase 3
• Small C-terminal domain phosphatase 3
• YA22

Background

Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation (By similarity). Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CTDSPL