CTDSP1 Antibody

Immunohistochemistry-Paraffin: CTDSP1 Antibody [NBP1-06029] - Staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CTDSP1 Antibody Summary

• Immunogen: Peptide with sequence C-RGKGDQKSAASQK corresponding to internal region according to NP_872580.1.
• Epitope: C-RGKGDQKSAASQK
• Specificity: This antibody is expected to recognise both reported isoforms (NP_067021.1and NP_872580.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CTDSP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave an approx. 60 kDa band in human spleen, lung and placenta lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 29.2 kDa band according to NP_067021.1 and 29.1 kDa band according to NP_872580.1. The 60 kDa band was successfully blocked by incubation with the immunizing peptide.
• Control:

  CTDSP1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CTDSP1 Antibody

• carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
• CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) smallphosphatase 1
• NIF3
• NLI-IF
• NLIIFEC 3.1.3.16
• NLI-interacting factor 3
• SCP1Nuclear LIM interactor-interacting factor 3
• Small CTD phosphatase 1
• Small C-terminal domain phosphatase 1

Background

Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in theC-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase IItranscription, possibly by controlling the transition from initiation/capping to processive transcript elongation.Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronalcells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CTDSP1 Antibody (NBP1-06029)(1)

Publication using NBP1-06029

• Zhang Y, Kim Y, Genoud N et al. Determinants for dephosphorylation of the RNA polymerase II C-terminal domain by Scp1. Mol Cell 2006-12-08 [PMID: 17157258]

Bioinformatics

• Gene Symbol: CTDSP1
• Entrez:
  • 58190(Human)
  • 227292(Mouse)
  • 363249(Rat)
• Uniprot:
  • NP_067021.1(Human)