CSK Antibody

Images

 
Western Blot: CSK Antibody [NB100-808] - (1ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CSK Antibody [NB100-808] - Staining (0.1ug/ml) of paraffin embedded Human Tonsil. Microwaved antigen retrieval with the interfollicular areas. B) High magnification view of germinal centre ...read more
Immunohistochemistry-Paraffin: CSK Antibody [NB100-808] - (0.1ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining of germinal ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CSK Antibody Summary

Immunogen
Peptide with sequence C-EQLEHIKTHELHL corresponding to C-Terminus according to NP_004374.1, NP_001120662.1.
Localization
Nuclear
Specificity
Reported variants represent identical protein: NP_004374.1; NP_001120662.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CSK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 50 kDa band observed in lysates of Jurkat and in mouse and rat spleen lysates (calculated MW of 50.7 kDa band according to NP_004374.1). IHC-P: Human colon shows staining of a cell layer between the mucosal columns and the muscularis mucosae.
Positive Control
Jurkat Lysate (NB800-PC2)
CSK Lysate (NBL1-09525)
Publications
Read Publication using NB100-808.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CSK Antibody

  • C-Src kinase
  • c-src tyrosine kinase
  • EC 2.7.10
  • EC 2.7.10.2
  • MGC117393
  • Protein-tyrosine kinase CYL
  • tyrosine-protein kinase CSK

Background

C terminal Src kinase (CSK) is a non receptor protein tyrosine kinase. One of its major functions is to specifically phosphorylate a conserved C terminal tyrosine of Src family kinases thereby down regulating their kinase activity. CSK is expressed ubiquitously as a protein of approximately 50 KDa in cytosol, but a fraction distributes to lipid rafts due to interaction with the lipid raft associated protein PAG (CSK binding protein).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CSK Antibody (NB100-808)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CSK
Entrez
Uniprot