CSK Antibody (CSK-04)

Images

 
Immunocytochemistry/ Immunofluorescence: CSK Antibody (CSK-04) [NB500-344] - Staining of Csk in human HeLa cell line using anti-Csk (CSK-04; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IP
Clone
CSK-04
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CSK Antibody (CSK-04) Summary

Immunogen
Bacterially expressed recombinant fragment of human Csk corresponding to amino acids 330-450.
Localization
Nuclear
Specificity
The antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa non-receptor protein tyrosine kinase expressed in cytosol.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CSK
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50
Application Notes
Immunocytochemistry: Immunosorbent (CSK-04 antibody covalently bound to beads) immunoprecipitates Csk from cell lysates. In WB predicted molecular weight 54 kDa. Weak non-specific band of cca 60 kDa is detected in human cell lines in lower antibody dilutions.
Positive Control
CSK Lysate (NBL1-09525)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CSK Antibody (CSK-04)

  • C-Src kinase
  • c-src tyrosine kinase
  • EC 2.7.10
  • EC 2.7.10.2
  • MGC117393
  • Protein-tyrosine kinase CYL
  • tyrosine-protein kinase CSK

Background

C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases that are localized in lipid rafts is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CSK Antibody (NB500-344) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-344AF405
Alexa Fluor 488 Labeled NB500-344AF488
Alexa Fluor 647 Labeled NB500-344AF647
Alexa Fluor 700 Labeled NB500-344AF700
DyLight 350 Labeled NB500-344UV
DyLight 405 Labeled NB500-344V
DyLight 488 Labeled NB500-344G
DyLight 680 Labeled NB500-344FR
DyLight 755 Labeled NB500-344IR

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Bioinformatics

Gene Symbol CSK
Entrez