CSE1L/CAS/Exportin-2 Antibody

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Ramos human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CSE1L/CAS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5288) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CSE1L/CAS at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CSE1L/CAS/Exportin-2 Antibody Summary

• Immunogen: E. coli-derived recombinant human CSE1L
  Ala739-His933
  Accession # P55060
• Specificity: Detects endogenous human, mouse, and rat CSE1L in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CSE1L
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CSE1L/CAS/Exportin-2 Antibody

• CAS
• CASMGC117283
• Cellular apoptosis susceptibility protein
• chromosome segregation 1 (yeast homolog)-like
• Chromosome segregation 1-like protein
• CSE1 chromosome segregation 1-like (yeast)
• CSE1
• CSE1L
• exp2
• exportin-2
• Importin-alpha re-exporter
• MGC130037
• XPO2
• XPO2MGC130036

Background

CSE1L (Chromosome segregation 1-like protein; also known as Exportin-2 (XPO2) and Cellular Apoptosis Susceptibility (CAS)) is a 110 kDa member of the XPO2/CSE1 family of proteins. It is expressed in divergent proliferating cell types such as colon and mammary epithelium, fibroblasts, hepatocytes, and spermatogonia. It participates in the export of importin-alpha from the nucleus, and regulates p53-sensitive gene transcription. Human CSE1L is 971 amino acids (aa) in length and contains an N-terminal importin domain (aa 29-102) and a DNA-association region (aa 372-385). There are multiple splice variants. There is a six aa substitution for aa 190-971, separate deletions of aa 133-341 and 257-312, an alternate start site at Met235 with a deletion of aa 350-517, and an alternate start site at Met100 with a 25 aa substitution for aa 674 - 971. Over aa 739-933, human CSE1L shows 100% aa identity to mouse CSE1L.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSE1L
• Uniprot:
  • P55060(Human)