CRHR1/CRF1 Antibody

Immunohistochemistry-Paraffin: CRHR1/CRF1 Antibody [NBP1-02328] - Staining of Human pars intermedia. Concentration 20 ug/ml.

Product Discontinued

CRHR1/CRF1 Antibody Summary

• Immunogen: Synthetic 15 amino acid peptide from N-terminal extracellular domain of human CRHR1/CRF1.
• Specificity: Human CRF1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except CRHR2 (73%).
• Predicted Species: Porcine (93%), Bovine (93%), Opossum (93%), Bat (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRHR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 15-20 ug/ml
• Application Notes: IHC: NBP1-02328 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Reactivity Notes

Gorilla, Gibbon, Marmoset. Predicted cross-reactivity based on sequence identity: Panda (100%), Elephant (87%), Sheep (80%), Lizard (80%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CRHR1/CRF1 Antibody

• CHR-R1
• corticotropin releasing hormone receptor 1
• corticotropin-releasing factor receptor 1
• corticotropin-releasing factor type 1 receptor
• Corticotropin-releasing hormone receptor 1
• CRF1
• CRFR
• CRF-R
• CRFR1
• CRF-R1
• CRF-R-1
• CRFR-1
• CRHR1
• CRH-R-1
• CRHR1f
• CRH-R1h
• CRHR1L
• CRHRCRH-R1
• seven transmembrane helix receptor

Background

Corticotropin-releasing factor receptor 1 (CRFR1/CRHR1), a Corticotropin-Releasing Factor Receptor, is involved in mediating the stress response and anxiety-related behaviors. Mutations of the receptor reduce anxiety-related behaviors and may be involved in the pathogenesis of Cushing disease. Multiple isoforms of CRFR1 are produced by alternative splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRHR1
• Entrez:
  • 1394(Human)
• Uniprot:
  • P34998(Human)