CRABP2 Antibody

Western Blot: CRABP2 Antibody [NB100-2590] - Whole cell lysate (5, 15 and 50 ug) from MCF7 cells. Antibody used at 0.04 ug/ml.

Immunohistochemistry-Paraffin: CRABP2 Antibody [NB100-2590] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CRABP2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, IHC, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CRABP2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 80 and the C-terminus (residue 138) of human Cellular Retinoic Acid Binding Protein 2 using the numbering given in entry NP_001869.1 (GeneID 1382 ).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRABP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:2000-1:10000
• Application Notes: Prepare working dilution immediately before use. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  CRABP2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CRABP2 Antibody

• cellular retinoic acid binding protein 2
• Cellular retinoic acid-binding protein II
• CRABP2
• CRABP-II
• CRABP-IIcellular retinoic acid-binding protein 2
• RBP6

Background

A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The inducibility of the CRABP2 gene suggests that this isoform is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRABP2 Antibody (NB100-2590)(2)

Publications using NB100-2590

• Design, Synthesis and Biological Evaluation of Nuclear Receptor-degradation Inducers. Itoh Y, Kitaguchi R, Ishikawa M et al. Bioorg Med Chem [PMID: 22014751] (WB, Human)
• Itoh Y, Ishikawa M, Kitaguchi R et al. Development of Target Protein-Selective Degradation Inducer for Protein Knockdown. Bioorganic & Medicinal Chemistry. 2011-01-01 [PMID: 21515062]

Bioinformatics

• Gene Symbol: CRABP2
• Entrez:
  • 1382(Human)
• Uniprot:
  • P29373()