CRABP1 Antibody (1A1)

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Western Blot: CRABP1 Antibody (AT1A1) [NBP1-37070] - The extracts of mouse eye (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CRABP1 (1:500). Proteins were visualized using a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
1A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CRABP1 Antibody (1A1) Summary

Immunogen
human CRABP1 (1-137aa) purified from E. coli
Specificity
This antibody is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CRABP1 protein.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CRABP1
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:100-1:2000
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500-1000.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CRABP1 Antibody (1A1)

  • cellular retinoic acid binding protein 1
  • CRABP
  • CRABPI
  • CRABP-Icellular retinoic acid-binding protein 1
  • RBP5Cellular retinoic acid-binding protein I

Background

CRABP1 (cellular retinoic acid-binding protein 1) is a member of specific carrier proteins for members of the vitamin A family. CRABP1 is assumed to play an important role in retinoic acid-mediated differentiation and proliferation processes. CRABP1 is structurally similar to the cellular retinol-binding proteins, but binds only retinoic acid. It is constitutively expressed and is believed to have different functions in the cell than the related CRABP2. Interestingly, Sytenol A bakuchiol has very specific receptor specificity over retinol and has no effect on the RAR-beta and RAR-gamma receptors and down-regulates CRABP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CRABP1
Entrez
Uniprot