Human CPN1 ELISA Kit (Colorimetric)

ELISA: Human CPN1 ELISA Kit (Colorimetric) [NBP3-27420] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Standard Curve Range: 0.312 - 20 ng/mL (example only; lot dependent)
• Sensitivity: 0.107 ng/mL (example only; lot dependent)

Human CPN1 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 0.312 - 20 ng/mL (example only; lot dependent)
• Sensitivity: 0.107 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CPN1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human CPN1 ELISA Kit (Colorimetric)

• ACBP
• Anaphylatoxin inactivator
• Arginine carboxypeptidase
• carboxypeptidase N polypeptide 1 50 kD
• Carboxypeptidase N polypeptide 1
• Carboxypeptidase N small subunit
• carboxypeptidase N, polypeptide 1
• carboxypeptidase N, polypeptide 1, 50kD
• CPN
• CPN1
• CPN-1
• CPNcarboxypeptidase N catalytic chain
• EC 3.4.17
• EC 3.4.17.3
• FLJ40792
• kininase I
• Kininase-1
• Lysine carboxypeptidase
• Plasma carboxypeptidase B
• SCPN
• SCPNcarboxypeptidase N catalytic subunit
• Serum carboxypeptidase N

Background

Carboxypeptidase N (arginine carboxypeptidase or CPN) cleaves basic amino acid residues from the carboxy terminal of peptides and proteins. The enzyme plays a central role in regulating the biologic activity of peptides such as kinins and anaphylatoxins, and therefore is also known as kininase-1 and anaphylatoxin inactivator. CPN is a tetrameric complex consisting of two identical regulatory subunits (CPN reg) and two identical catalytic subunits (CPN cat). The two glycosylated CPN reg subunits protect the two CPN cat subunits and keep them in the circulation. CPN reg is a member of the leucinerich repeat family of proteins and the gene which encodes CPN reg maps to human chromosome 8p22-p23. CPN cat is a member of the regulatory B-type carboxypeptidase group and the gene which encodes CPN cat maps to human chromosome 10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for CPN1 ELISA Kit (NBP3-27420). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CPN1