CPI17 alpha Antibody (3A7) - Azide and BSA Free

Western Blot: CPI17 alpha Antibody (3A7) [H00094274-M01] - Analysis of PPP1R14A expression in transfected 293T cell line by PPP1R14A monoclonal antibody (M01), clone 3A7.Lane 1: PPP1R14A transfected lysate(16.7 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: CPI17 alpha Antibody (3A7) [H00094274-M01] - Detection limit for recombinant GST tagged PPP1R14A is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CPI17 alpha Antibody (3A7) - Azide and BSA Free Summary

• Immunogen: PPP1R14A (AAH21089, 1 a.a. ~ 147 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAQRLGKRVLSKLQSPSRARGPGGSPGGLQKRHARVTVKYDRRELQRRLDVEKWIDGRLEELYRGMEADMPDEINIDELLELESEEERSRKIQGLLKSCGKPVEDFIQELLAKLQGLHRQPGLRQPSPSHDGSLSPLQDRARTAHP
• Specificity: PPP1R14A - protein phosphatase 1, regulatory (inhibitor) subunit 14A
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPP1R14A
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CPI17 alpha Antibody (3A7) - Azide and BSA Free

• 17 kDa PKC-potentiated inhibitory protein of PP1
• CPI-1717-kDa PKC-potentiated inhibitory protein of PP1
• CPI1717-KDa protein
• PKC-potentiated inhibitory protein of PP1
• PPP1INL
• Protein kinase C-potentiated inhibitor protein of 17 kDa
• protein phosphatase 1 regulatory subunit 14A
• protein phosphatase 1, regulatory (inhibitor) subunit 14A

Background

PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase (see MIM 603768). Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2+) concentration.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP1R14A
• Entrez:
  • 94274(Human)
• OMIM:
  • 608153(Human)
• Uniprot:
  • AAH21089(Human)