COX4-I1 Antibody (673803) [Alexa Fluor® 532]

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clone: 673803
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

COX4-I1 Antibody (673803) [Alexa Fluor® 532] Summary

• Immunogen: E.coli-derived recombinant human COX4-I2
  Asn39-Lys171
  Accession # Q96KJ9
• Specificity: Detects human COX4-I2 in direct ELISAs. In Western blots, 100% cross-reactivity with recombinant human (rh) COX4-I1 and
  no cross-reactivity with rhCOX-1 or rhCOX-2 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for COX4-I1 Antibody (673803) [Alexa Fluor® 532]

• COX IV-1
• COX4
• COX4-1
• COX4I1
• COX4-I1
• COXIV
• COXIV-
• Cytochrome c oxidase polypeptide IV
• cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• cytochrome c oxidase subunit IV
• MC4DN16

Background

COX4-I2 (Cytochrome c oxidase subunit 4) is a 21-22 kDa member of the Cytochrome c oxidase IV family of proteins and is predominantly expressed in lung. It is a component of COX, an inner mitochondrial membrane multimeric dimer that catalyzes the transfer of electrons from Cytochrome c to dioxygen. COX4-I2 is induced following blockade of mitochondrial respiration, leading to increased production of reactive oxygen species and necrosis. The ubiquitously expressed COX4-I1 is the product of a different gene and shares only 50% aa identity with COX4-I2. Within aa 39-171 (the oxidase domain), human COX4-I2 shares 84% and 81% aa sequence identity with mouse and rat COX4-I2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.