HAF007). For addtional reference, recombinant human COX4-I1 (5 ng/lane) and recombinant human COX4-I2 (2.5 ng/lane) were included. A specific band was detected for COX4 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human COX4 Monoclonal Antibody (Catalog # MAB6980) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For addtional reference, recombinant human COX4-I1 (5 ng/lane) and recombinant human COX4-I2 (2.5 ng/lane) were included. A specific band was detected for COX4 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6980-SP | |||
![Western Blot: COX IV Isoform 2 Antibody (1F2) [H00084701-M01] - Analysis of COX4I2 expression in transfected 293T cell line by COX4I2 monoclonal antibody (M01), clone 1F2.Lane 1: COX4I2 transfected lysate(20 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/COX-IV-Isoform-2-Antibody-1F2-Western-Blot-H00084701-M01-img0007.jpg "Western Blot: COX IV Isoform 2 Antibody (1F2) [H00084701-M01] - Analysis of COX4I2 expression in transfected 293T cell line by COX4I2 monoclonal antibody (M01), clone 1F2.Lane 1: COX4I2 transfected lysate(20 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: COX IV Isoform 2 Antibody (1F2) [H00084701-M01] - Analysis of monoclonal antibody to COX4I2 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/COX-IV-Isoform-2-Antibody-1F2-Immunohistochemistry-Paraffin-H00084701-M01-img0004.jpg)
![Immunoprecipitation: COX IV Isoform 2 Antibody (1F2) [H00084701-M01] - Analysis of COX4I2 transfected lysate using anti-COX4I2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with COX4I2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/COX-IV-Isoform-2-Antibody-1F2-Immunoprecipitation-H00084701-M01-img0005.jpg)
![ELISA: COX IV Isoform 2 Antibody (1F2) [H00084701-M01] - Detection limit for recombinant GST tagged COX4I2 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/COX-IV-Isoform-2-Antibody-1F2-ELISA-H00084701-M01-img0008.jpg)
NL007) and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for 
NL007) and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for ![Immunohistochemistry: COX IV Isoform 2 Antibody - BSA Free [NBP3-47540] - Immunohistochemistry of paraffin-embedded human ovary tumor using (COX IV Isoform 2 antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-47540_rabbit-cox-iv-isoform-2-pab-bsa-free-28420251133322.jpg "Immunohistochemistry: COX IV Isoform 2 Antibody - BSA Free [NBP3-47540] - Immunohistochemistry of paraffin-embedded human ovary tumor using (COX IV Isoform 2 antibody) at dilution of 1:50")
![Immunoprecipitation: COX IV Isoform 2 Antibody - BSA Free [NBP3-47540] - IP Result of anti-COX IV Isoform 2 (IP:, 3ug; Detection: 1:1000) with HepG2 cells lysate 8000ug.](http://images.novusbio.com/fullsize/nbp3-47540_rabbit-cox-iv-isoform-2-pab-bsa-free-284202511402779.jpg)
![Western Blot: COX IV Isoform 2 Antibody - BSA Free [NBP3-47540] - MCF7 cells were subjected to SDS PAGE followed by western blot with (COX IV Isoform 2 antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-47540_rabbit-cox-iv-isoform-2-pab-bsa-free-28420251141487.jpg)
![Immunohistochemistry-Paraffin: COX IV Isoform 2 Antibody [NBP3-13152] - COX IV Isoform 2 antibody detects COX IV Isoform 2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. COX IV Isoform 2 stained by COX IV Isoform 2 antibody (NBP3-13152) diluted at 1:500. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/COX-IV-Isoform-2-Antibody-Immunohistochemistry-Paraffin-NBP3-13152-img0002.jpg "Immunohistochemistry-Paraffin: COX IV Isoform 2 Antibody [NBP3-13152] - COX IV Isoform 2 antibody detects COX IV Isoform 2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. COX IV Isoform 2 stained by COX IV Isoform 2 antibody (NBP3-13152) diluted at 1:500. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min")
![Western Blot: COX IV Isoform 2 Antibody [NBP1-44068] - Analysis of COX IV Isoform 2 in Human Skeletal Muscle lysate (35ug protein in RIPA buffer) using NBP1-44068 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/COX-IV-Isoform-2-Antibody-Western-Blot-NBP1-44068-img0001.jpg "Western Blot: COX IV Isoform 2 Antibody [NBP1-44068] - Analysis of COX IV Isoform 2 in Human Skeletal Muscle lysate (35ug protein in RIPA buffer) using NBP1-44068 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![SDS-Page: Recombinant Human COX IV Isoform 2 Protein [H00084701-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00084701-P01-1.jpg "SDS-Page: Recombinant Human COX IV Isoform 2 Protein [H00084701-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: COX IV Isoform 2 Overexpression Lysate (Native) [NBP2-05989] - COX IV Isoform 2 Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for COXIV Isoform 2.](http://images.novusbio.com/fullsize/COX-IV-Isoform-2-Overexpression-Lysate-Native-Western-Blot-NBP2-05989-img0002.jpg "Western Blot: COX IV Isoform 2 Overexpression Lysate (Native) [NBP2-05989] - COX IV Isoform 2 Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for COXIV Isoform 2.")
![Western Blot: COX IV Isoform 2 Antibody [H00084701-D01P] - Analysis of COX4I2 expression in transfected 293T cell line by COX4I2 polyclonal antibody.Lane 1: COX4I2 transfected lysate(20.00 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/COX-IV-Isoform-2-Antibody-Western-Blot-H00084701-D01P-img0002.jpg "Western Blot: COX IV Isoform 2 Antibody [H00084701-D01P] - Analysis of COX4I2 expression in transfected 293T cell line by COX4I2 polyclonal antibody.Lane 1: COX4I2 transfected lysate(20.00 KDa).Lane 2: Non-transfected lysate.")
