Corneodesmosin Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 647

Corneodesmosin Antibody [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Corneodesmosin
  Lys33-Pro529 (Ser153 del)
  Accession # NP_001255
• Specificity: Detects human Corneodesmosin in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Corneodesmosin Antibody [Alexa Fluor® 647]

• CDSN
• Corneodesmosin
• D6S586E
• differentiated keratinocyte S protein
• HTSS
• S protein
• S

Background

Corneodesmosin, also known as CDSN and the S gene product, is a highly polymorphic secreted glycoprotein that plays an important structural role in the skin (1). It is expressed by differentiated keratinocytes in the corneal layer of the skin and is a major component of corneodesmosomes (2-4). It is also expressed in the inner root sheath of hair follicles (5, 6). Corneodesmosome has a high content of glycine, serine, and proline residues that promote its folding into a series of Gly-loop domains (2, 7). Corneodesmosin forms oligomers and associates homophilically to strengthen the adhesion between corneocytes (8, 9). Corneodesmosin-deficient mice exhibit a detachment of the corneal layer of the skin as well as hypotrichosis of the scalp and baldness (6, 10). Corneodesmosin is secreted by keratinocytes as a 52-56 kDa molecule which is then subjected to repeated sequential N- and C-terminal proteolysis (11). Species of 46, 43, 36, and 15 kDa are present in corneocytes (7, 11). Cleavage of the N-terminal Gly-loop diminishes Corneodesmosin’s ability to mediate adhesion, and this is a prerequisite for normal desquamation of the skin (8, 9). Reduced proteolysis of Corneodesmosin in psoriasis lesions is associated with the persistence of corneodesmosomes and scale retention (12). Premature truncation of Corneodesmosin is associated with hypotrichosis of the scalp (13).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.