COQ3 Antibody

Western Blot: COQ3 Antibody [NBP1-88724] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Immunocytochemistry/Immunofluorescence: COQ3 Antibody [NBP1-88724] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.

Immunohistochemistry-Paraffin: COQ3 Antibody [NBP1-88724] - Staining of human liver.

Immunohistochemistry-Paraffin: COQ3 Antibody [NBP1-88724] - Staining of human kidney shows strong cytoplasmic positivity, with a granular pattern in cells in tubules.

Immunohistochemistry-Paraffin: COQ3 Antibody [NBP1-88724] - Staining of human pancreas shows low expression as expected.

Immunohistochemistry-Paraffin: COQ3 Antibody [NBP1-88724] - Staining of human parathyroid gland shows high expression.

Immunohistochemistry-Paraffin: COQ3 Antibody [NBP1-88724] - Staining in human parathyroid gland and pancreas tissues using anti-COQ3 antibody. Corresponding COQ3 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: COQ3 Antibody [NBP1-88724] - Staining of human colon, heart muscle, kidney and liver using Anti-COQ3 antibody NBP1-88724 (A) shows similar protein distribution across tissues to independent antibody NBP1-88725 (B).

Immunohistochemistry-Paraffin: COQ3 Antibody [NBP1-88724] - Staining of human kidney.

Immunohistochemistry-Paraffin: COQ3 Antibody [NBP1-88724] - Staining of human heart muscle.

Immunohistochemistry-Paraffin: COQ3 Antibody [NBP1-88724] - Staining of human colon.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

COQ3 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: WEKFVSPETLESILESNGLSVQTVVGMLYNPFSGYWHWSENTSLNYAAHAVKSRVQEHPASAEFVLKGETEELQANACTNPAVHEKLKK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COQ3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  COQ3 Protein (NBP1-88724PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for COQ3 Antibody

• 3-demethylubiquinone-10 3-methyltransferase
• bA9819.1
• coenzyme Q3 homolog, methyltransferase (S. cerevisiae)
• coenzyme Q3 homolog, methyltransferase (yeast)
• DHHB methyltransferase
• DHHBMT
• DHHB-MT
• DHHBMTASE
• DHHB-MTase
• Dihydroxyhexaprenylbenzoate methyltransferase
• EC 2.1.1
• EC 2.1.1.114
• EC 2.1.1.64
• hexaprenyldihydroxybenzoate methyltransferase, mitochondrial3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase
• methyltransferase COQ3
• UG0215E05

Background

Ubiquinone, also known as coenzyme Q, or Q, is a critical component of the electron transport pathways of both eukaryotes and prokaryotes (Jonassen and Clarke, 2000 [PubMed 10777520]). This lipid consists of a hydrophobic isoprenoid tail and a quinone head group. The tail varies in length depending on the organism, but its purpose is to anchor coenzyme Q to the membrane. The quinone head group is responsible for the activity of coenzyme Q in the respiratory chain. The S. cerevisiae COQ3 gene encodes an O-methyltransferase required for 2 steps in the biosynthetic pathway of coenzyme Q. This enzyme methylates an early coenzyme Q intermediate, 3,4-dihydroxy-5-polyprenylbenzoic acid, as well as the final intermediate in the pathway, converting demethyl-ubiquinone to coenzyme Q. The COQ3 gene product is also capable of methylating the distinct prokaryotic early intermediate 2-hydroxy-6-polyprenyl phenol.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COQ3