COPE Overexpression Lysate (Native)

Images

 
Western Blot: COPE Overexpression Lysate (Adult Normal) [NBL1-09378] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for COPE.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

COPE Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203531 and based on accession number NM_007263. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens coatomer protein complex, subunit epsilon (COPE), transcript variant 1, mRNA.
Gene
COPE

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
34.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for COPE

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for COPE Overexpression Lysate (Native)

  • coatomer epsilon subunit
  • coatomer protein complex, subunit epsilon
  • coatomer subunit epsilon
  • epsilon coat protein
  • Epsilon-coat protein
  • epsilon-COP
  • FLJ13241

Background

The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longest transcript, and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for COPE Lysate (NBL1-09378) (0)

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol COPE