COP Antibody (3E10)

Sandwich ELISA: COP Antibody (3E10) [H00114769-M01] - Detection limit for recombinant GST tagged COP1 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

COP Antibody (3E10) Summary

• Immunogen: COPl (NP_443121, 1 a.a. ~ 97 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MADKVLKEKRKLFIHSMGEGTINGLLDELLQTRVLNQEEMEKVKRENATVMDKTRALIDSVIPKGAQACQICITYICEEDSYLAETLGLSAGPIPGN
• Specificity: COP1 - CARD only protein
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CARD16
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for COP Antibody (3E10)

• CARD only domain-containing protein 1
• CARD only protein
• caspase recruitment domain family, member 16
• Caspase-1 inhibitor COP
• COP1caspase-1 dominant-negative inhibitor pseudo-ICE
• COPcaspase recruitment domain-containing protein 16
• EC 3.4.22.36
• Pseudo interleukin-1 beta converting enzyme
• pseudo interleukin-1beta converting enzyme
• PSEUDO-ICE

Background

Caspase inhibitor. Acts as a regulator of procaspase-1/CASP1 activation implicated in the regulation of theproteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release ofIL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro-inflammatory cytokineresponse. Also able to inhibit CASP1-mediated neuronal cell death, TNF-alpha, hypoxia-, UV-, andstaurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspasesCASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CARD16
• Entrez:
  • 114769(Human)
• Uniprot:
  • NP_443121(Human)