Contactin-5 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Contactin-5 Antibody [NBP1-83243] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Immunohistochemistry-Paraffin: Contactin-5 Antibody [NBP1-83243] - Staining of human testis shows strong cytoplasmic positivity in leydig cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Contactin-5 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: IVVICSAEGEPSAAPTDVKATSVSVSEILVAWKHIKESLGRPQGFEVGYWKDMEQEDTAETVKTRGNESFVILTGLEGNTLYHFTVRAYNGAGYG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CNTN5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot Reported in reported in scientific literature (PMID: 30747104)
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Contactin-5 Protein (NBP1-83243PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Contactin-5 Antibody - BSA Free

• CNTN5
• contactin 5
• Contactin5
• Contactin-5
• hNB-2
• HNB-2s
• MGC163491
• NB2
• NB-2
• neural adhesion molecule
• Neural recognition molecule NB-2

Background

CNTN5 is encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.; ; Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity in the cerebral cortical neurons but not in hippocampal neurons. Involved in neuronal activity in the auditory system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Contactin-5 Antibody (NBP1-83243)(1)

Publication using NBP1-83243

• Deneault E, Faheem M, White SH et al. CNTN5- /+ or EHMT2- /+ human iPSC-derived neurons from individuals with autism develop hyperactive neuronal networks Elife 2019-02-12 [PMID: 30747104] (WB, Human)

Bioinformatics

• Gene Symbol: CNTN5