Contactin-3 Antibody [Alexa Fluor® 488]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

Contactin-3 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Contactin-3
  Glu20-Thr1003
  Accession # Q07409
• Specificity: Detects human, mouse, and rat Contactin-3 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human Contactin-5 and recombinant mouse Contactin-4 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Contactin-3 Antibody [Alexa Fluor® 488]

• BIG-1
• CNTN3
• contactin 3 (plasmacytoma associated)
• Contactin3
• Contactin-3
• PANG
• PCS

Background

Contactin-3 (also BIG-1 in rat and PANG in mouse) is a 150 kDa member of the TAG/F3 subgroup, contactin family, Ig superfamily hierarchy. It is a GPI linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons and olfactory granule cells. Its exact function is unclear, but it likely mediates cell-cell interaction and may promote neurite outgrowth. Human Contactin-3 preproprecursor is 1028 amino acids (aa) in length. It contains a 19 aa signal sequence, a 983 aa mature region and a 26 aa cleavable C-terminal prosegment. The mature region shows six C2- type IG like domains (aa 26-593), followed by four fibronectin type III modules (aa 598-992). There is one potential alternate start site at Met128. Over aa 26-992, human Contactin-3 shares 92% aa identity with mouse Contactin‑3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.