Connexin 50/GJA8 Antibody - Azide and BSA Free

Western Blot: Connexin 50/GJA8 Antibody [NBP3-15748] - Western blot analysis of extracts of Raji cells, using Connexin 50/GJA8 antibody (NBP3-15748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunocytochemistry/Immunofluorescence: Connexin 50/GJA8 Antibody [NBP3-15748] - Immunofluorescence analysis of L929 cells using Connexin 50/GJA8 antibody (NBP3-15748) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: Connexin 50/GJA8 Antibody [NBP3-15748] - Immunofluorescence analysis of C6 cells using Connexin 50/GJA8 antibody (NBP3-15748) at dilution of 1:100. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Connexin 50/GJA8 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Connexin 50/GJA8 Antibody - Azide and BSA Free (NBP3-15748) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 234-433 of human Connexin 50/GJA8 (NP_005258.2). SALKRPVEQPLGEIPEKSLHSIAVSSIQKAKGYQLLEEEKIVSHYFPLTEVGMVETSPLPAKPFNQFEEKISTGPLGDLSRGYQETLPSYAQVGAQEVEGEGPPAEEGAEPEVGEKKEEAERLTTEEQEKVAVPEGEKVETPGVDKEGEKEEPQSEKVSKQGLPAEKTPSLCPELTTDDARPLSRLSKASSRARSDDLTV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GJA8
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for Connexin 50/GJA8 Antibody - Azide and BSA Free

• alpha 8, 50kD (connexin 50)
• CAE
• CAE1
• Connexin 50
• Connexin-50
• CX50
• CZP1
• gap junction alpha-8 protein
• gap junction membrane channel protein alpha-8
• gap junction protein, alpha 8, 50kDa (connexin 50)
• gap junction protein, alpha 8, 50kDa
• GJA8
• lens intrinsic membrane protein MP70
• MP70

Background

The 433 amino acid long, 48 kDA transmembrane connexin, gap junction alpha-8 protein encoded by the GJA8 gene is critical in growth and development of lens fiber cells. Mutations in the GJA8 gene may lead to zonular pulverulent cataracts, nuclear progressive cataracts, and cataract-microcornea syndrome. The GJA8 gene has been linked to myopia, microphthalmia, neuronitis, cycstic fibrosis, neuopathy, cervical adenocarcinoma, and juvenile myoclonic epilepsy. The GJA8 gene interacts with TJP1, MIP, MED14, GJA1, and GJB1 to participate in pathways such as gap junction assembly, membrane trafficking, and connexin synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: GJA8