Connexin 43/GJA1 Antibody (3E5)

Western Blot: Connexin 43/GJA1 Antibody (3E5) [H00002697-M01] - GJA1 monoclonal antibody (M01), clone 3E5. Analysis of GJA1 expression in human placenta.

Product Discontinued

Connexin 43/GJA1 Antibody (3E5) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GJA1 (AAH26329, 261 a.a. ~ 361 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVD*
• Marker: Gap Junctions Marker
• Specificity: GJA1 - gap junction protein, alpha 1, 43kDa (connexin 43)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GJA1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Connexin 43/GJA1 Antibody (3E5)

• Connexin 43
• connexin-43
• CX43
• CX43gap junction protein, alpha-like
• DFNB38
• Gap junction 43 kDa heart protein
• gap junction alpha-1 protein
• gap junction protein, alpha 1, 43kDa (connexin 43)
• gap junction protein, alpha 1, 43kDa
• GJA1
• GJAL
• HSS
• ODD
• ODDD
• ODOD
• SDTY3

Background

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Connexin 43/GJA1 Antibody (H00002697-M01). (Showing 1 - 1 of 1 FAQ).

1. Can I use Connexin 43/GJA1 Antibody (3E5) in immunohistochemistry for rat ?
   • I have taken a look at this particular clone however we have not tested this in IHC or in rat. The homology with rat is 97.0% which is good however we do not know if this will work in IHC. Please see this antibody NBP1-67530. This has been validated for both IHC and Rat. Another product, NB100-91717, has been validated for Rat and IHC-Paraffin.

Bioinformatics

• Gene Symbol: GJA1
• Entrez:
  • 2697(Human)
• OMIM:
  • 121(Human)
• Uniprot:
  • AAH26329(Human)