Complexin-1/2 Antibody (837802) [Unconjugated]

Western blot shows lysates of human brain (cerebellum) tissue, mouse brain (cortex) tissue, and rat brain (cerebellum) tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Complexin-1/2 Monoclonal Antibody (Catalog # MAB8069) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Complexin-1/2 at approximately 17-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Complexin-1/2 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human/Mouse/Rat Complexin-1/2 Monoclonal Antibody (Catalog # MAB8069) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.5 mg/mL. A specific band was detected for Complexin-1/2 at approximately 23 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse/Rat Complexin-1/2 Monoclonal Antibody (Catalog # MAB8069). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC
• Clone: 837802
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Complexin-1/2 Antibody (837802) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Complexin-1
  Met1-Lys134
  Accession # O14810
• Specificity: Detects human Complexin-1 and Complexin-2 in ELISAs. Detects human, mouse, and rat Complexin-1 and -2 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complexin-1/2 Antibody (837802) [Unconjugated]

• Complexin-1/2
• CPLX1;CPX1;CPX-I;DEE63;EIEE63

Background

Complexin-1 and Complexin-2 are cytosolic proteins that belongs to the complexin/synaphin family. They are expressed in select neurons and pancreatic b-cells. Complexin-1 serves as a block against premature neurotransmitter/insulin granule release. It does so by binding to the SNARE complex that contains SNAP25, VAMP2 and STX1A, generating oligomers of SNARE complexes that are optimally oriented relative to the positions of vesicle and plasma membranes. This effectively places synaptic vesicles in an optimal configuration, allowing for a coordinated, uniform fusion and release upon appropriate stimulus. Typically, complexin-1 is described as a clamp that blocks (vesicle) fusion, and the stimulus that overcomes it is one that induces an influx of synaptotagmin-detectable calcium. Both human complexin-1 and complexin-2 are 134 amino acids (aa) in length. Full-length human complexin-1 shares 84% aa sequence identity with human complexin-2. Human and mouse complexin-1 share 97% aa sequence identity, while human and mouse complexin-2 are 100% identical.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O14810(Human)