Complexin-2 Antibody (837802) [Alexa Fluor® 488]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 837802
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Complexin-2 Antibody (837802) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human Complexin-1
  Met1-Lys134
  Accession # O14810
• Specificity: Detects human Complexin-1 and Complexin-2 in ELISAs. Detects human, mouse, and rat Complexin-1 and -2 in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complexin-2 Antibody (837802) [Alexa Fluor® 488]

• complexin 2
• Complexin II
• Complexin-2
• CPLX2
• CPX II
• CPX2
• CPX-2
• CPX-II
• DKFZp547D155
• Hfb1
• MGC138492
• Synaphin 1
• synaphin 1,921-L
• synaphin-1

Background

Complexin-1 and Complexin-2 are cytosolic proteins that belongs to the complexin/synaphin family. They are expressed in select neurons and pancreatic b-cells. Complexin-1 serves as a block against premature neurotransmitter/insulin granule release. It does so by binding to the SNARE complex that contains SNAP25, VAMP2 and STX1A, generating oligomers of SNARE complexes that are optimally oriented relative to the positions of vesicle and plasma membranes. This effectively places synaptic vesicles in an optimal configuration, allowing for a coordinated, uniform fusion and release upon appropriate stimulus. Typically, complexin-1 is described as a clamp that blocks (vesicle) fusion, and the stimulus that overcomes it is one that induces an influx of synaptotagmin-detectable calcium. Both human complexin-1 and complexin-2 are 134 amino acids (aa) in length. Full-length human complexin-1 shares 84% aa sequence identity with human complexin-2. Human and mouse complexin-1 share 97% aa sequence identity, while human and mouse complexin-2 are 100% identical.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.