Complement Factor MASP3/MASP1 Antibody (4H2A9)

• Reactivity: Hu
• Applications: ELISA
• Clone: 4H2A9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Complement Factor MASP3/MASP1 Antibody (4H2A9) Summary

• Immunogen: CCP-1 domain of MASP-1
• Specificity: The monoclonal antibody 4H2A9 reacts with the CCP1 domain, which is shared by MASP-1, MASP-3 and MAp-44.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MASP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
• Application Notes: The monoclonal antibody 4H2A9 is suitable for western blotting and immunoprecipitation experiments. Moreover, the antibody can be used in immunoassays detecting MASP-1, MASP-3 and/or MAp44. For Immunoassay dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for Complement Factor MASP3/MASP1 Antibody (4H2A9)

• Complement Factor MASP3
• Complement factor MASP-3
• Complement-activating component of Ra-reactive factor
• CRARF1
• CRARFMAP1
• DKFZp686I01199
• EC 3.4.21
• EC 3.4.21.-
• EC 3.4.21.104
• EC 3.4.21.42
• FLJ26383
• mannan-binding lectin serine peptidase 1 (C4/C2 activating component ofRa-reactive factor)
• mannan-binding lectin serine protease 1 (C4/C2 activating component ofRa-reactive factor)
• mannan-binding lectin serine protease 1
• Mannose-binding lectin-associated serine protease 1
• Mannose-binding protein-associated serine protease
• MAp44
• MASP
• MASP1
• MASP-1
• MASP3
• MGC126283
• MGC126284
• PRSS5
• Ra-reactive factor serine protease p100
• RaRF
• Serine protease 5

Background

The monoclonal antibody 4H2A9 reacts with the CCP1 domain, which is shared by MASP-1, MASP-3 and MAp-44. In total 5 MASP proteins have been identified (MASP-1, MASP-2, MASP-3, MAp19 and MAp44) which are encoded by 2 genes. The MASP-2 gene gives rise to MASP2 and MAp19 due to alternative splicing, whereas the MASP-1 gene encodes a primary transcript which can be spliced to three different mRNAs coding for MASP-1, MASP-3 and MAp44 having molecular weights of 88, 89 and 44kDa, respectively. On the protein level MASP-1 and MASP-2 are shown to be responsible for complement activation through proteolytic cleavage of C2 and C4, whereas MASP-3 has been suggested to inhibit the generation of the C3 convertase. So far the role of the truncated proteases MAp44 and Map19 remain unknown. The monoclonal antibody 4H2A9 is suitable for western blotting and immunoprecipitation experiments. Moreover, the antibody can be used in immunoassays detecting MASP-1, MASP-3 and/or MAp44.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MASP1