Complement Factor MASP3/MASP1 Antibody (1E2)

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 1E2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Complement Factor MASP3/MASP1 Antibody (1E2) Summary

• Immunogen: The antibody recognizes the heavy chain common to both MASP-1 and MASP-3.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MASP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
  • Immunoprecipitation
  • Western Blot
• Application Notes: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. The antibody does not work in Western blotting of reduced samples.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for Complement Factor MASP3/MASP1 Antibody (1E2)

• Complement Factor MASP3
• Complement factor MASP-3
• Complement-activating component of Ra-reactive factor
• CRARF1
• CRARFMAP1
• DKFZp686I01199
• EC 3.4.21
• EC 3.4.21.-
• EC 3.4.21.104
• EC 3.4.21.42
• FLJ26383
• mannan-binding lectin serine peptidase 1 (C4/C2 activating component ofRa-reactive factor)
• mannan-binding lectin serine protease 1 (C4/C2 activating component ofRa-reactive factor)
• mannan-binding lectin serine protease 1
• Mannose-binding lectin-associated serine protease 1
• Mannose-binding protein-associated serine protease
• MAp44
• MASP
• MASP1
• MASP-1
• MASP3
• MGC126283
• MGC126284
• PRSS5
• Ra-reactive factor serine protease p100
• RaRF
• Serine protease 5

Background

Three pathways of complement activation have been reported: the antibody-dependent classical pathway, the antibody-independent alternative pathway and the lectin pathway. Activation of each pathway involves formation of serine protease complexes, which results in activation of the central complement component C3. In the lectin pathway, mannose binding-lectin (MBL)-associated serine proteases (MASP) form complexes with polymeric lectin molecules which are involved in pattern recognition. Upon binding of the recognition molecules to carbohydrates on the surface of microorganisms, MASP are converted to their active forms and initiate complement activation. Three types of human MASP have been reported. MASP-1, MASP-2 and MASP-3. MASP-1 appears to cleave the second complement component C2, but not C4. The proteolytic activities of MASP-1 are inhibited by C1-inhibitor. Furthermore MASP-1 has a reactivity profile very similar to that of thrombin. MASP-1 is able to catalyse the formation of cross-linked fibrin. Participation of MASP-1 in cross-linked fibrin clot formation causes release of a chemotactic factor representing a biologically significant activity of MASP-1. The alternative-splicing product from MASP-1 gene is called MASP-3. MASP-1 is associated with smaller MBL oligomers whereas MASP-3 is found on larger oligomers. The substrate of MASP-3 is unknown. The antibody recognizes the heavy chain common to both MASP-1 and MASP-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MASP1