Human Complement Factor I ELISA Kit (Colorimetric)

ELISA: Human Complement Factor I ELISA Kit (Colorimetric) [NBP2-60565] - Standard curve.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Culture, Saliva, Milk
• Standard Curve Range: 0.375 - 24 ug/mL (example only; lot dependent)
• Sensitivity: 0.26 ug/ml (example only; lot dependent)

Human Complement Factor I ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 0.375 - 24 ug/mL (example only; lot dependent)
• Sensitivity: 0.26 ug/ml (example only; lot dependent)
• Assay Type: Competitive ELISA
• Inter-Assay: CV% < 9.20% (example only; lot dependent)
• Intra-Assay: CV% < 4.80% (example only; lot dependent)
• Spike Recovery: 96.00% (example only; lot dependent)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CFI

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Complement Factor I ELISA Kit (Colorimetric)

• AHUS3
• C3b Inactivator
• C3B/C4B inactivator
• C3BINA
• C3b-inactivator
• CFI
• complement component I
• complement control protein factor I
• complement factor I heavy chain
• Complement Factor I
• EC 3.4.21
• EC 3.4.21.45
• FIC3b-INA
• I factor (complement)
• IF
• KAF
• Konglutinogen-activating factor
• light chain of factor I

Background

Factor I is a plasma protein of molecular weight 88 kDa, consisting of two disulphide linked chains of 50 and 38 kDa. Factor I is a serine protease with a high degree of specificity for C3b and C4b. It has a requirement for protein cofactors for cleavage of these complement proteins, Factor H, CR1 or MCP are required for C3b cleavage and C4bp or CR1 for C4b cleavage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Complement Factor I ELISA Kit (NBP2-60565). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CFI