COMMD1 Antibody (762203) [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 762203
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

COMMD1 Antibody (762203) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human COMMD1
  Ser37-Ser135
  Accession # Q8N668
• Specificity: Detects human COMMD1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Attractin or rhCaspr1 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for COMMD1 Antibody (762203) [Alexa Fluor® 750]

• C2orf5
• COMM domain-containing protein 1
• COMMD1
• copper metabolism (Murr1) domain containing 1
• copper metabolism gene MURR1
• MGC27155
• MURR1
• MURR1chromosome 2 open reading frame 5 (MURR1)
• Protein Murr1

Background

COMMD1, also known as MURR1 and the copper toxicosis gene product, is a ubiquitously expressed 21 kDa copper binding protein. It contains an N-terminal domain (aa 1-121) and C-terminal domain (aa 125-190) which are separated by a protease sensitive site. It forms high molecular weight oligomeric complexes both in solution and in association with phospholipid membranes. COMMD1 is found in the nucleus and cytoplasm as well as in endocytic vesicle membrane fractions. It binds and regulates the activity of a variety of proteins including the copper transporter Wilson disease protein (ATP7B), the delta ENaC epithelial cell sodium channel, the cystic fibrosis transmembrane regulator (CFTR), superoxide dismutase 1 (SOD1), XIAP, HIF-1 alpha, and Cullin RING ubiquitin ligases. COMMD1 promotes the ubiquitination and degradation of the RelA subunit of NFkB, thereby inhibiting its chromatin association and nuclear targeting as well as the replication of HIV in resting T cells. Within aa 37-135, human COMMD1 shares 94% aa sequence identity with mouse and rat COMMD1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.