Collagen XXIII alpha 1 Antibody (468642) [Unconjugated]

Western blot shows lysates of NCI-H460 human large cell lung carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human Collagen XXIII a1 Monoclonal Antibody (Catalog # MAB4165) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Collagen XXIII a1 at approximately 75 and 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Collagen XXIII a1 was detected in immersion fixed paraffin-embedded sections of human adenocarcinoma using Human Collagen XXIII a1 Monoclonal Antibody (Catalog # MAB4165) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 468642
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Collagen XXIII alpha 1 Antibody (468642) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Collagen XXIII alpha 1
  Glu111-Lys540
  Accession # NP_775736
• Specificity: Detects human Collagen XXIII alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 50% cross-reactivity with recombinant human Collagen XXV alpha 1 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: COL23A1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Collagen XXIII alpha 1 Antibody (468642) [Unconjugated]

• COL23A1
• collagen alpha-1(XXIII) chain
• Collagen XXIII alpha 1
• collagen, type XXIII, alpha 1
• DKFZp434K0621
• procollagen, type XXIII, alpha 1

Background

Collagen XXIII alpha 1 (COL23A1) is an epithelial cell type 2 transmembrane glycoprotein with multiple collagen like domains in its extracellular region. Intracellular furin mediated cleavage of the 68 kDa molecule generates a 58 kDa shed fragment that forms disulfide linked trimers. An alternately spliced isoform consists of nearly 300 amino acids of the membrane proximal extracellular domain (ECD), flanked by substituted sequences. COL23A1 is upregulated in many transformed cell lines and serves as an indicator for tumor aggressiveness in prostate cancer. Within the ECD, human COL23A1 shares 89% aa sequence identity with mouse and rat COL23A1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen XXIII alpha 1 Antibody (MAB4165)(3)

Publications using MAB4165

• T. Manon‐Jensen, S. Sun, M. Lindholm, V. Domislović, P. Giuffrida, M. Brinar et al. Elevated ectodomain of type 23 collagen is a novel biomarker of the intestinal epithelium to monitor disease activity in ulcerative colitis and Crohn's disease United European Gastroenterology Journal 3/29/2021 [PMID: 33351719]
• Fujiang Xu, Kun Chang, Jian Ma, Yuanyuan Qu, Huyang Xie, Bo Dai et al. The Oncogenic Role of COL23A1 in Clear Cell Renal Cell Carcinoma Scientific Reports 8/29/2017 [PMID: 28852123]
• Spivey KA, Chung I, Banyard J A role for collagen XXIII in cancer cell adhesion, anchorage-independence and metastasis. Oncogene, 2011-10-03;31(18):2362-72. 2011-10-03 [PMID: 21963851] (ICC, Western Blot, Human)

Bioinformatics

• Gene Symbol: COL23A1
• Uniprot:
  • NP_775736()