Human Collagen XIII alpha 1 ELISA Kit (Colorimetric)

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ELISA: Human Collagen XIII alpha 1 ELISA Kit (Colorimetric) [NBP2-75831] - Samples were spiked with high concentrations of Human Collagen XIII alpha 1 and diluted with Reference Standard & Sample Diluent to produce ...read more
ELISA: Human Collagen XIII alpha 1 ELISA Kit (Colorimetric) [NBP2-75831] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

Order Details

Human Collagen XIII alpha 1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human COL13 in samples. No significant cross-reactivity or interference between Human COL13 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.16%
Intra-Assay
CV% < 4.94%
Spike Recovery
85-103%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
COL13A1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Collagen XIII alpha 1 ELISA Kit (Colorimetric)

  • COL13A1
  • collagen alpha-1(XIII) chain
  • Collagen XIII alpha 1
  • collagen, type XIII, alpha 1
  • FLJ42485

Background

COL13A1 encodes the alpha chain of one of the nonfibrillar collagens. The function of this gene product is not known, however, it has been detected at low levels in all connective tissue-producing cells so it may serve a general function in connective tissues. Unlike most of the collagens, which are secreted into the extracellular matrix, collagen XIII contains a transmembrane domain and the protein has been localized to the plasma membrane. The transcripts for this gene undergo complex and extensive splicing involving at least eight exons. Like other collagens, collagen XIII is a trimer; it is not known whether this trimer is composed of one or more than one alpha chain isomer. A number of alternatively spliced transcript variants have been described, but the full length nature of some of them has not been determined. Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol COL13A1