Human Collagen VIII alpha 1 ELISA Kit (Colorimetric)

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ELISA: Human Collagen VIII alpha 1 ELISA Kit (Colorimetric) [NBP2-75884] - Samples were spiked with high concentrations of Human Collagen VIII alpha 1 and diluted with Reference Standard & Sample Diluent to produce ...read more
ELISA: Human Collagen VIII alpha 1 ELISA Kit (Colorimetric) [NBP2-75884] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

Order Details

Human Collagen VIII alpha 1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human COL8a1 in samples. No significant cross-reactivity or interference between Human COL8a1 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.21%
Intra-Assay
CV% < 5.01%
Spike Recovery
85-105%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
COL8A1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Collagen VIII alpha 1 ELISA Kit (Colorimetric)

  • alpha-1 polypeptide
  • chromosome 3 open reading frame 7
  • collagen, type VIII, alpha 1
  • smooth muscle cell-expressed and macrophage conditioned medium-induced proteinsmag-64

Background

The Collagen VIII alpha 1 gene encodes one of the two alpha chains of type VIII collagen. The gene product is a short chain collagen and amajor component of the basement membrane of the corneal endothelium. The type VIII collagen fibril can be either ahomo- or a heterotrimer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol COL8A1