Recombinant Human COL4A3BP His Protein

SDS-Page: COL4A3BP Protein [NBP1-44465] - CERT, 33.1 kDa (290aa) confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human COL4A3BP His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 347-598 of Human COL4A3BP
  
  

  Source: E.coli

  Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMLH WPTSLPSGDA FSSVGTHRFV QKVEEMVQNH MTYSLQDVGG DANWQLVVEE GEMKVYRREV EENGIVLDPL KATHAVKGVT GHEVCNYFWN VDVRNDWETT IENFHVVETL ADNAIIIYQT HKRVWPASQR DVLYLSVIRK IPALTENDPE TWIVCNFSVD HDSAPLNNRC VRAKINVAMI CQTLVSPPEG NQEISRDNIL CKITYVANVN PGGWAPASVL RAVAKREYPK FLKRFTSYVQ EKTAGKPILF

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CERT1
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 32.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human COL4A3BP His Protein

• Ceramide transfer protein
• CERTFLJ20597
• CERTL
• collagen type IV alpha-3-binding protein
• collagen, type IV, alpha 3 (Goodpasture antigen) binding protein
• Goodpasture antigen-binding protein
• GPBPSTARD11ceramide transporter
• hCERT
• lipid-transfer protein CERTL
• StARD11
• StAR-related lipid transfer (START) domain containing 11
• StAR-related lipid transfer protein 11
• START domain containing 11
• START domain-containing protein 11

Background

CERT is a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Recombinant human CERT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for COL4A3BP Recombinant Protein (NBP1-44465). (Showing 1 - 1 of 1 FAQ).

1. I am assaying this protein by UV spectroscopy. Can you tell me its molar extinction coefficient at 280 nm?
   • Unfortunately this is not information that has been measured by our lab.

Bioinformatics

• Gene Symbol: CERT1