COG7 Antibody

Western Blot: COG7 Antibody [NBP1-05032] - (0.03ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: COG7 Antibody [NBP1-05032] - (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

COG7 Antibody Summary

• Immunogen: Peptide with sequence C-KTRPEDYRQVSK corresponding to internal region according to NP_705831.1.
• Epitope: internal region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: COG7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.03-0.1 ug/ml
• Application Notes: Western blot: Approx. 85kDa band observed in lysates cell lines HeLa, Jurkat and MOLT4 (calculated MW of 86.3kDa according to NP_705831.1). Primary incubation was 1 hour.
  IHC: Paraffin embedded Human Testis.
• Control:

  COG7 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for COG7 Antibody

• CDG2E
• component of oligomeric golgi complex 7COG complex subunit 7
• conserved oligomeric Golgi complex subunit 7

Background

Multiprotein complexes are key determinants of Golgi apparatus structure and its capacity for intracellular transport and glycoprotein modification. Several complexes have been identified, including the Golgi transport complex (GTC), the LDLC complex, which is involved in glycosylation reactions, and the SEC34 complex, which is involved in vesicular transport. These 3 complexes are identical and have been termed the conserved oligomeric Golgi (COG) complex, which includes COG7 (Ungar et al., 2002 [PubMed 11980916]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for COG7 Antibody (NBP1-05032)(1)

Publication using NBP1-05032

• Oka T, Vasile E, Penman M et al. Genetic analysis of the subunit organization and function of the conserved oligomeric golgi (COG) complex: studies of COG5- and COG7-deficient mammalian cells. J Biol Chem 2005-09-23 [PMID: 16051600]

Bioinformatics

• Gene Symbol: COG7
• Entrez:
  • 91949(Human)
• Uniprot:
  • NP_705831.1(Human)