![Western Blot: Cofilin [p Ser3] Antibody [NBP3-05394] - Western blot analysis of extracts of NIH/3T3 cells, using Cofilin pAb (NBP3-05394) at 1:1000 dilution or CFL1 antibody (A1704).NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Cofilin-[p-Ser3]-Antibody-Western-Blot-NBP3-05394-img0003.jpg "Western Blot: Cofilin [p Ser3] Antibody [NBP3-05394] - Western blot analysis of extracts of NIH/3T3 cells, using Cofilin pAb (NBP3-05394) at 1:1000 dilution or CFL1 antibody (A1704).NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Immunogen | A synthetic phosphorylated peptide around S3 of human Cofilin (NP_005498.1). MASGV |
| Modification | p Ser3 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CFL1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 18 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cofilin Antibody (NBP3-05394)Find related products by research area.
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| Gene Symbol | CFL1 |
![Immunocytochemistry/Immunofluorescence: Cofilin [p Ser3] Antibody [NBP3-05394] - Immunofluorescence analysis of methanol-fixed HeLa cells using Cofilin antibody (NBP3-05394).](http://images.novusbio.com/fullsize/Cofilin-[p-Ser3]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05394-img0002.jpg "Immunocytochemistry/Immunofluorescence: Cofilin [p Ser3] Antibody [NBP3-05394] - Immunofluorescence analysis of methanol-fixed HeLa cells using Cofilin antibody (NBP3-05394).")
![Immunohistochemistry-Paraffin: Cofilin [p Ser3] Antibody [NBP3-05394] - Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Cofilin antibody (NBP3-05394).](http://images.novusbio.com/fullsize/Cofilin-[p-Ser3]-Antibody-Immunohistochemistry-Paraffin-NBP3-05394-img0001.jpg "Immunohistochemistry-Paraffin: Cofilin [p Ser3] Antibody [NBP3-05394] - Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Cofilin antibody (NBP3-05394).")
![Immunocytochemistry/ Immunofluorescence: Cofilin [p Ser3] Antibody - Azide and BSA Free [Cofilin] - Immunofluorescence analysis of methanol-fixed HeLa cells using Cofilin Rabbit pAb .](http://images.novusbio.com/fullsize/nbp3-05394_rabbit-polyclonal-cofilin-p-ser3-antibody-5220251638020.jpg "Immunocytochemistry/ Immunofluorescence: Cofilin [p Ser3] Antibody - Azide and BSA Free [Cofilin] - Immunofluorescence analysis of methanol-fixed HeLa cells using Cofilin Rabbit pAb .")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")