Synthetic peptide corresponding to amino acids 154-166 of human cofilin with N-terminal cysteine added, conjugated to maleimide-activated keyhole limpet hemocyanin (KLH). The corresponding immunizing sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin.
Cytoplasmic and Nuclear
Detects a band at approximately 19kDa. Reacts with muscle and non-muscle cofilin.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
10mM PBS (pH 7.4)
15mM Sodium Azide
Western Blot 1:10000
This antibody can be used in Western Blot. For WB use whole extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, mouse NIH/3T3, and dog MDCK kidney cells. IF only recommended for indirect staining. Staining of cofilin is inhibited by the immunizing peptide. Also suitable for microarray. Not tested in other applications. *Optimal dilutions should be determined by the end user.
Read Publications using NB100-1900 in the following applications:
Cofilin is a small phosphoinositide sensitive, actin binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their 'pointed' (minus) ends. Cofilin binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in an apparent pH-dependent, Ca2+ independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. Cofilin is ubiquitous in tissues of eukaryotes and is especially abundant in neuronal tissues. It is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.