![Western Blot: Cofilin Antibody [NB100-1900] - Whole extract of A-431 Human epithelial carcinoma cells were separated on SDS-PAGE and probed with Anti-Cofilin antibody produced in Rabbit. The antibody was developed using 1:6000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat.Lanes:1. 1:10,000 antibody2. 1:20,000 antibody3. Negative Control](http://images.novusbio.com/fullsize/Cofilin-Antibody-Western-Blot-NB100-1900-img0002.jpg "Western Blot: Cofilin Antibody [NB100-1900] - Whole extract of A-431 Human epithelial carcinoma cells were separated on SDS-PAGE and probed with Anti-Cofilin antibody produced in Rabbit. The antibody was developed using 1:6000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat.Lanes:1. 1:10,000 antibody2. 1:20,000 antibody3. Negative Control")
| Reactivity | Hu, Mu, Rt, Po, CaSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF, MA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit Cofilin Antibody - BSA Free (NB100-1900) is a polyclonal antibody validated for use in WB, ICC/IF and Simple Western. Anti-Cofilin Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Synthetic peptide corresponding to amino acids 154-166 of human cofilin with N-terminal cysteine added, conjugated to maleimide-activated keyhole limpet hemocyanin (KLH). The corresponding immunizing sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin. |
| Localization | Cytoplasmic and Nuclear |
| Specificity | Detects a band at approximately 19kDa. Reacts with muscle and non-muscle cofilin. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CFL1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This antibody can be used in Western Blot. For WB use whole extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, mouse NIH/3T3, and dog MDCK kidney cells. IF only recommended for indirect staining. Staining of cofilin is inhibited by the immunizing peptide. Also suitable for microarray. Not tested in other applications. *Optimal dilutions should be determined by the end user. See Simple Western Antibody Database for Simple Western validation |
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| Theoretical MW | 19 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 10mM PBS (pH 7.4) |
| Preservative | 0.09% Sodium Azide |
| Purity | IgG purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
ICC | Canine | 07/18/2022 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cofilin Antibody (NB100-1900)Find related products by research area.
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ACTB - an abundant cytoskeletal component with applications for gene expression analysis Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: Cofilin Antibody [NB100-1900] - NIH-3T3 Mouse fibroblasts cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.2% Triton X-100. Fixed cells were stained with 1:1,000 Anti-Cofilin antibody produced in Rabbit . The antibody was developed using 1:100 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat. Picture B: cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/Cofilin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1900-img0001.jpg "Immunocytochemistry/Immunofluorescence: Cofilin Antibody [NB100-1900] - NIH-3T3 Mouse fibroblasts cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.2% Triton X-100. Fixed cells were stained with 1:1,000 Anti-Cofilin antibody produced in Rabbit . The antibody was developed using 1:100 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat. Picture B: cells were counterstained with DAPI (blue) to stain nuclei.")
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