Cofilin Antibody

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, CaSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Cofilin Antibody Summary

Immunogen
Synthetic peptide corresponding to amino acids 154-166 of human cofilin with N-terminal cysteine added, conjugated to maleimide-activated keyhole limpet hemocyanin (KLH). The corresponding immunizing sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin.
Localization
Cytoplasmic and Nuclear
Specificity
Detects a band at approximately 19kDa. Reacts with muscle and non-muscle cofilin.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CFL1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Immunocytochemistry/Immunofluorescence 1:1000
Application Notes
This antibody can be used in Western Blot. For WB use whole extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, mouse NIH/3T3, and dog MDCK kidney cells. IF only recommended for indirect staining. Staining of cofilin is inhibited by the immunizing peptide. Also suitable for microarray. Not tested in other applications. *Optimal dilutions should be determined by the end user.
Positive Control
Cofilin Lysate (NBL1-09126)
Publications
Read Publications using
NB100-1900 in the following applications:

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
15mM Sodium Azide
Purity
IgG purified

Alternate Names for Cofilin Antibody

  • 18 kDa phosphoprotein
  • CFL
  • cofilin 1 (non-muscle)
  • Cofilin, non-muscle isoform
  • cofilin-1
  • p18

Background

Cofilin is a small phosphoinositide sensitive, actin binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their 'pointed' (minus) ends. Cofilin binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in an apparent pH-dependent, Ca2+ independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. Cofilin is ubiquitous in tissues of eukaryotes and is especially abundant in neuronal tissues. It is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cofilin Antibody (NB100-1900)(2)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(2)
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Human
(1)
Rat
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cofilin Antibody (NB100-1900) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Cofilin Antibody (NB100-1900)

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Blogs on Cofilin.

ACTB - an abundant cytoskeletal component with applications for gene expression analysis
Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi...  Read full blog post.

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Bioinformatics

Gene Symbol CFL1
Entrez
Uniprot