Coagulation Factor II/Thrombin Antibody

Western Blot: Prothrombin Antibody [H00002147-B01P] - Analysis of F2 expression in transfected 293T cell line by F2 polyclonal antibody. Lane 1: F2 transfected lysate(68.42 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Prothrombin Antibody [H00002147-B01P] - Analysis of purified antibody to F2 on HepG2 cell. (antibody concentration 10 ug/ml)

Product Discontinued

Coagulation Factor II/Thrombin Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: F2 (NP_000497.1, 1 a.a. - 622 a.a.) full-length human protein. MAHVRGLQLPGCLALAALCSLVHSQHVFLAPQQARSLLQRVRRANTFLEEVRKGNLERECVEETCSYEEAFEALESSTATDVFWAKYTACETARTPRDKLAACLEGNCAEGLGTNYRGHVNITRSGIECQLWRSRYPHKPEINSTTHPGADLQENFCRNPDSSTTGPWCYTTDPTVRRQECSIPVCGQDQVTVAMTPRSEGSSVNLSPPLEQCVPDRGQQYQGRLAVTTHGLPCLAWASAQAKALSKHQDFNSAVQLVENFCRNPDGDEEGVWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDGRIVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDIALMKLKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKETWTANVGKGQPSVLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDQFGE
• Specificity: coagulation factor II (thrombin)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: F2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against mammalian transfected lysate in WB, ELISA and IF.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Coagulation Factor II/Thrombin Antibody

• coagulation factor II (thrombin) receptor-like 2
• Coagulation factor II receptor-like 2 (protease-actovated receptor 3)
• Coagulation factor II receptor-like 2
• Coagulation Factor II
• F2
• PAR-3
• PAR3proteinase-activated receptor 3
• protease-activated receptor 3
• proteinase-activated receptor-3
• PT
• serine protease
• Thrombin receptor-like 2
• Thrombin

Background

Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prothrombin Antibody (H00002147-B01P)(1)

Publication using H00002147-B01P

• Huang YC, Wu YR, Tseng MY et al. Increased Prothrombin, Apolipoprotein A-IV, and Haptoglobin in the Cerebrospinal Fluid of Patients with Huntington's Disease. PLoS One. 2011-01-31 [PMID: 21297956]

FAQs for Prothrombin Antibody (H00002147-B01P). (Showing 1 - 1 of 1 FAQ).

1. Do you have neutralizing antibodies for anti-mouse Thrombin?
   • We currently have 1 neutralizing Thrombin antibody (NBP1-95029), however it is an anti-human antibody and has only been validated in human samples. If you would like to test this antibody in mouse samples, you may be interested in participating in our Innovators Reward Program.

Bioinformatics

• Gene Symbol: F2
• Entrez:
  • 2147(Human)
• Uniprot:
  • NP_000497.1(Human)