![Immunohistochemistry-Paraffin: CNOT2 Antibody [NBP1-30454] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CNOT used at a dilution of 1:1,000 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/CNOT2-Antibody-Immunohistochemistry-Paraffin-NBP1-30454-img0009.jpg "Immunohistochemistry-Paraffin: CNOT2 Antibody [NBP1-30454] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CNOT used at a dilution of 1:1,000 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .")
| Reactivity | HuSpecies Glossary |
| Applications | IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit CNOT2 Antibody - BSA Free (NBP1-30454) is a polyclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen for this product maps to a region between residue 490 and 540 of human CCR4-NOT transcription complex, subunit 2 using the numbering given in entry NP_055330.1 (GeneID 4848). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CNOT2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CNOT2 |
![Immunohistochemistry-Paraffin: CNOT2 Antibody [NBP1-30454] - FFPE section of human colon carcinoma. Antibody used at a dilution of 1:250 Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/CNOT2-Antibody-Immunohistochemistry-Paraffin-NBP1-30454-img0010.jpg "Immunohistochemistry-Paraffin: CNOT2 Antibody [NBP1-30454] - FFPE section of human colon carcinoma. Antibody used at a dilution of 1:250 Detection: DAB staining using Immunohistochemistry Accessory Kit.")
![Immunohistochemistry-Paraffin: CNOT2 Antibody [NBP1-30454] - FFPE section of human ovarian carcinoma. Antibody used at a dilution of 1:250 Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/CNOT2-Antibody-Immunohistochemistry-Paraffin-NBP1-30454-img0008.jpg "Immunohistochemistry-Paraffin: CNOT2 Antibody [NBP1-30454] - FFPE section of human ovarian carcinoma. Antibody used at a dilution of 1:250 Detection: DAB staining using Immunohistochemistry Accessory Kit.")
![Immunoprecipitation: CNOT2 Antibody [NBP1-30454] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CNOT2 antibody NBP1-30454 used for IP at 10 mcg/mg lysate.](http://images.novusbio.com/fullsize/CNOT2-Antibody-Immunoprecipitation-NBP1-30454-img0003.jpg "Immunoprecipitation: CNOT2 Antibody [NBP1-30454] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CNOT2 antibody NBP1-30454 used for IP at 10 mcg/mg lysate.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")