CLPP Antibody (3E2) - Azide and BSA Free

Western Blot: CLPP Antibody (3E2) [H00008192-M01] - CLPP monoclonal antibody (M01), clone 3E2. Analysis of CLPP expression in PC-12.

Immunohistochemistry-Paraffin: CLPP Antibody (3E2) [H00008192-M01] - Analysis of monoclonal antibody to CLPP on formalin-fixed paraffin-embedded human kidney. Antibody concentration 1 ug/ml.

Western Blot: CLPP Antibody (3E2) [H00008192-M01] - CLPP monoclonal antibody (M01), clone 3E2 Analysis of CLPP expression in A-431.

Sandwich ELISA: CLPP Antibody (3E2) [H00008192-M01] - Detection limit for recombinant GST tagged CLPP is 1 ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clone: 3E2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CLPP Antibody (3E2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CLPP (NP_006003, 178 a.a. ~ 277 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HQPSGGARGQATDIAIQAEEIMKLKKQLYNIYAKHTKQSLQVIESAMERDRYMSPMEAQEFGILDKVLVHPPQDGEDEPTLVQKEPVEAAPAAEPVPAST
• Specificity: CLPP - ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLPP
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA and IHC-P.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34345994). .

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CLPP Antibody (3E2) - Azide and BSA Free

• ATP-dependent protease ClpAP, proteolytic subunit, human
• ClpP (caseinolytic protease, ATP-dependent, proteolytic subunit, E. coli)homolog
• ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E.coli)
• ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog (E.coli)
• ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog
• EC 3.4.21.92
• Endopeptidase Clp
• putative ATP-dependent Clp protease proteolytic subunit, mitochondrial

Background

The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CLPP Antibody (H00008192-M01)(2)

Publications using H00008192-M01

• Maletzko A, Key J, Wittig I Et al. Increased presence of nuclear DNAJA3 and upregulation of cytosolic STAT1 and of nucleic acid sensors trigger innate immunity in the ClpP-null mouse Neurogenetics 2021-08-03 [PMID: 34345994] (Mouse)
• Kita K, Suzuki T, Ochi T. Diphenylarsinic acid promotes degradation of glutaminase C by mitochondrial Lon protease. J Biol Chem. 2012-04-05 [PMID: 22493432]

Bioinformatics

• Gene Symbol: CLPP
• Entrez:
  • 8192(Human)
• OMIM:
  • 601119(Human)
• Uniprot:
  • NP_006003(Human)