CLK3 Antibody (1F10)

Western Blot: CLK3 Antibody (1F10) [H00001198-M05] - Analysis of CLK3 expression in transfected 293T cell line by CLK3 monoclonal antibody (M05), clone 1F10. Lane 1: CLK3 transfected lysatE (58.6 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: CLK3 Antibody (1F10) [H00001198-M05] - Analysis of monoclonal antibody to CLK3 on HeLa cell. Antibody concentration 8 ug/mL.

Immunohistochemistry-Paraffin: CLK3 Antibody (1F10) [H00001198-M05] - Analysis of monoclonal antibody to CLK3 on FFPE human placenta. Antibody concentration 0.5 ug/mL.

Western Blot: CLK3 Antibody (1F10) [H00001198-M05] - Analysis of CLK3 over-expressed 293 cell line, cotransfected with CLK3 Validated Chimera RNAi or non-transfected control. Blot probed with H00001198-M05. GAPDH (36.1 kDa) used as loading control.

Western Blot: CLK3 Antibody (1F10) [H00001198-M05] - Analysis of CLK3 expression in HeLa S3 NE (Cat # L013V3).

Western Blot: CLK3 Antibody (1F10) [H00001198-M05] - HeLa cell lysate. Antibody at 1:2000 in 5% BSA-TBST. Detection by Clarity Western ECL (BioRad). Image from verfied customer review.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, KD
• Clone: 1F10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CLK3 Antibody (1F10) Summary

• Immunogen: CLK3 (AAH02555, 36 a.a. ~ 136 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YPSRREPPPRRSRSRSHDRLPYQRRYRERRDSDTYRCEERSPSFGEDYYGPSRSRHRRRSRERGPYRTRKHAHHCHKRRTRSCSSASSRSQQSSKRSSRSV
• Specificity: CLK3 - CDC-like kinase 3
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLK3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein and cell lysate for WB. It has been used for IF, RNAi Validation, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CLK3 Antibody (1F10)

• CDC-like kinase 3
• CDC-like kinase 3PHCLK3
• CLK3
• dual specificity protein kinase CLK3
• EC 2.7.12.1
• FLJ22858
• PHCLK3/152

Background

This gene encodes a protein belonging to the serine/threonine type protein kinase family. This protein is a nuclear dual-specificity kinase that regulates the intranuclear distribution of the serine/arginine-rich (SR) family of splicing factors. Alternatively spliced transcript variants have been described for this gene, but their biological validity has not been determined. Pseudogenes located on chromosomes 1 and 9 have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for CLK3 Antibody (H00001198-M05) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Subha Dahal WB Human 01/03/2019

Summary

• Application: Western Blot
• Sample Tested: Hela Cell Lysate
• Species: Human
• Lot: 09243-1F10 WEPc

Comments

• Comments: Diluted CLK3 antibody (H00001198-M05) at 1:2000 in 5% BSA-TBST and probed the blot overnight at 4 degrees in shaker, followed by three 5 min washes in 1XTBST and probed with anti-Ms secondary at 1:5000, followed by three 5 min washes in 1XTBST and developed using Clarity Western ECL substrate from Bio-Rad. Shown is blot exposed for 7 s.

Bioinformatics

• Gene Symbol: CLK3
• Entrez:
  • 1198(Human)
• OMIM:
  • 602990(Human)
• Uniprot:
  • AAH02555(Human)