Recombinant Human CLIC4 His Protein

SDS-Page: Recombinant Human CLIC4 Protein [NBP1-51032] - 15 % SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human CLIC4 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-253 of Human CLIC4
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MALSMPLNGL KEEDKEPLIE LFVKAGSDGE SIGNCPFSQR LFMILWLKGV VFSVTTVDLK RKPADLQNLA PGTHPPFITF NSEVKTDVNK IEEFLEEVLC PPKYLKLSPK HPESNTAGMD IFAKFSAYIK NSRPEANEAL ERGLLKTLQK LDEYLNSPLP DEIDENSMED IKFSTRKFLD GNEMTLADCN LLPKLHIVKV VAKKYRNFDI PKEMTGIWRY LTNAYSRDEF TNTCPSDKEV EIAYSDVAKR LTK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CLIC4
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 30.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 0.1 M NaCl, 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human CLIC4 His Protein

• chloride intracellular channel 4 like
• chloride intracellular channel 4
• chloride intracellular channel protein 4
• CLIC4L
• DKFZp566G223
• FLJ38640
• H1
• huH1
• Intracellular chloride ion channel protein p64H1
• MTCLIC
• P64H1

Background

CLIC4 (chloride intracellular channel 4), also known as H1, CLIC4L or MTCLIC, is a 253 amino acid single-pass membrane protein that localizes to both the nucleus and the cytoplasm and contains one GST C-terminal domain. CLIC4 functions as a monomer that is able to form selective ion channels in target proteins, thereby facilitating the transport of chloride and other ions. CLIC4 is thought to play a role in apoptosis and has been shown to translocate to the nucleus under stress conditions. Recombinant human CLIC4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for CLIC4 Protein (NBP1-51032)(1)

Publication using NBP1-51032

• Lizano-Fallas V, del Amor A, Cristobal S Predictive toxicology of chemical mixtures using proteome-wide thermal profiling and protein target properties bioRxiv 2023-07-04

Bioinformatics

• Gene Symbol: CLIC4