CLEC12B Antibody (1062714) [Unconjugated]

PBMC monocytes were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CLEC12B Monoclonal Antibody (Catalog # MAB11476) or (B) isotype control antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 1062714
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CLEC12B Antibody (1062714) [Unconjugated] Summary

• Immunogen: Chinese Hamster Ovary cell line, CHO-derived human CLEC12B
  Leu65-Asp276
  Accession # Q2HXU8
• Specificity: Detects human CLEC12B in direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC12B Antibody (1062714) [Unconjugated]

• CLEC12B
• C-type lectin domain family 12 member B
• C-type lectin domain family 12, member B
• Macrophage Antigen H
• UNQ5782
• UNQ5782/PRO16089

Background

C-type lectin domain family 12 member B (CLEC12B) is a member of the C-type lectin-like family of proteins. CLEC12B is widely expressed at low levels in various human tissues except in the brain (1, 2). A truncated version lacking a portion of the carbohydrate-recognition domain (CRD) has been detected in mammary gland, lung and ovary, and was predicted to be nonfunctional (1). CLEC12B is a cell surface receptor that may play a role in viral recognition and modulate signaling cascades due to the presence of an ITIM motif within its cytoplasmic tail (1-3). Human CLEC12B is synthesized as a 276 amino acid (aa) protein that includes a 43 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 212 aa extracellular domain (ECD). Within the ECD, human CLEC12B shares 74% and 70% aa sequence identity with mouse and rat CLEC12B, respectively. The extracellular domain of CLEC12B shows considerable homology to the activating natural killer cell receptor NKG2D, and it antagonizes NKG2D mediated signaling through the ITIM motif (1). CLEC12B may be involved in limiting the activity of monocyte-derived immune cells after cell differentiation and possibly during inflammatory diseases. They play a role in HIV-1, mycobacterial, and Candida infections, and the coevolution of hosts and pathogens (4). Pathogen recognition by C-type lectins triggers signaling pathways that lead to the expression of specific cytokines which subsequently instruct adaptive T helper immune responses (4).

1. Hoffmann, S. et al. (2007) J. Biol. Chem. 282:22370.
2. Huysamen, C. et al. (2009) FEMS Microbiol. Lett. 290:121.
3. Monteiro, J.T. and B. Lepenies (2017) Viruses 9:59.
4. van den Berg, L.M. et al. (2012) Ann N Y Acad Sci. 1253:149.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q2HXU8()