CLEC-1 Antibody [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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CLEC-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC-1
Gln77-Asp280
Accession # Q8NC01
Specificity
Detects human CLEC-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human CLEC-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CLEC1A
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF1704 in the following application:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC-1 Antibody [Unconjugated]

  • CLEC1
  • CLEC-1
  • CLEC1A
  • CLEC1C-type lectin domain family 1 member A
  • C-type lectin domain family 1, member A
  • C-type lectin-like receptor 1
  • C-type lectin-like receptor-1
  • MGC34328

Background

CLEC-1 (C-type lectin-like receptor-1) is a type 2 transmembrane protein that belongs to the Dectin-1 family of C-type lectins (1, 2). It consists of a 52 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 207 aa extracellular domain (ECD) (3). The ECD contains a carbohydrate recognition domain (CRD) that includes cysteine residues with conserved spacing (4). In other C-type lectins, these cysteines form intrachain disulfide bonds that stabilize the CRD protein fold (4). CLEC-1 is a nonclassical C-type lectin in that its CRD lacks consensus amino acids which mediate calcium dependent carbohydrate binding in other C-type lectins (2, 3). The cytoplasmic domain contains one tyrosine, multiple serine and threonine, and a cluster of aspartic acid residues. The CLEC-1, CLEC-2, Dectin-1, and LOX-1 genes are located within the NK gene complex that also encodes several receptors involved in regulating NK cell activation (3, 5). CLEC-1 is most highly expressed in activated dendritic cells and at lower levels in endothelial cells and monocytes (3, 5). Within the CRD, human CLEC‑1 shares 65% aa sequence identity with mouse and rat CLEC-1 and 26%‑36% with the other human Dectin-1 family lectins CLEC-2, CLEC9A, CLEC12B, Dectin-1, LOX-1, and MICL.

  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Pyz, E. et al. (2006) Ann. Med. 38:242.
  3. Colonna, M. et al. (2000) Eur. J. Immunol. 30:697.
  4. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
  5. Sobanov, Y. et al. (2001) Eur. J. Immunol. 31:3493.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for CLEC-1 Antibody (AF1704) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1704:
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WB
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/05/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 22117783
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22117783

Details

Detection NotesSee PMID 22117783

Comments

CommentsPublished: See PMID 22117783

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Diseases for CLEC-1 Antibody (AF1704)

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Recent Reviews

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1
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Anonymous
01/05/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol CLEC1A
Entrez
Uniprot