CLEC12B Antibody (1062714) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: Flow
• Clone: 1062714
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

CLEC12B Antibody (1062714) [Alexa Fluor® 532] Summary

• Immunogen: Chinese Hamster Ovary cell line, CHO-derived human CLEC12B
  Leu65-Asp276
  Accession # Q2HXU8
• Specificity: Detects human CLEC12B in direct ELISA.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC12B Antibody (1062714) [Alexa Fluor® 532]

• CLEC12B
• C-type lectin domain family 12 member B
• C-type lectin domain family 12, member B
• Macrophage Antigen H
• UNQ5782
• UNQ5782/PRO16089

Background

C-type lectin domain family 12 member B (CLEC12B) is a member of the C-type lectin-like family of proteins. CLEC12B is widely expressed at low levels in various human tissues except in the brain (1, 2). A truncated version lacking a portion of the carbohydrate-recognition domain (CRD) has been detected in mammary gland, lung and ovary, and was predicted to be nonfunctional (1). CLEC12B is a cell surface receptor that may play a role in viral recognition and modulate signaling cascades due to the presence of an ITIM motif within its cytoplasmic tail (1-3). Human CLEC12B is synthesized as a 276 amino acid (aa) protein that includes a 43 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 212 aa extracellular domain (ECD). Within the ECD, human CLEC12B shares 74% and 70% aa sequence identity with mouse and rat CLEC12B, respectively. The extracellular domain of CLEC12B shows considerable homology to the activating natural killer cell receptor NKG2D, and it antagonizes NKG2D mediated signaling through the ITIM motif (1). CLEC12B may be involved in limiting the activity of monocyte-derived immune cells after cell differentiation and possibly during inflammatory diseases. They play a role in HIV-1, mycobacterial, and Candida infections, and the coevolution of hosts and pathogens (4). Pathogen recognition by C-type lectins triggers signaling pathways that lead to the expression of specific cytokines which subsequently instruct adaptive T helper immune responses (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.