CLEC12B Antibody (1062714) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: Flow
• Clone: 1062714
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

CLEC12B Antibody (1062714) [Alexa Fluor® 350] Summary

• Immunogen: Chinese Hamster Ovary cell line, CHO-derived human CLEC12B
  Leu65-Asp276
  Accession # Q2HXU8
• Specificity: Detects human CLEC12B in direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC12B Antibody (1062714) [Alexa Fluor® 350]

• CLEC12B
• C-type lectin domain family 12 member B
• C-type lectin domain family 12, member B
• Macrophage Antigen H
• UNQ5782
• UNQ5782/PRO16089

Background

C-type lectin domain family 12 member B (CLEC12B) is a member of the C-type lectin-like family of proteins. CLEC12B is widely expressed at low levels in various human tissues except in the brain (1, 2). A truncated version lacking a portion of the carbohydrate-recognition domain (CRD) has been detected in mammary gland, lung and ovary, and was predicted to be nonfunctional (1). CLEC12B is a cell surface receptor that may play a role in viral recognition and modulate signaling cascades due to the presence of an ITIM motif within its cytoplasmic tail (1-3). Human CLEC12B is synthesized as a 276 amino acid (aa) protein that includes a 43 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 212 aa extracellular domain (ECD). Within the ECD, human CLEC12B shares 74% and 70% aa sequence identity with mouse and rat CLEC12B, respectively. The extracellular domain of CLEC12B shows considerable homology to the activating natural killer cell receptor NKG2D, and it antagonizes NKG2D mediated signaling through the ITIM motif (1). CLEC12B may be involved in limiting the activity of monocyte-derived immune cells after cell differentiation and possibly during inflammatory diseases. They play a role in HIV-1, mycobacterial, and Candida infections, and the coevolution of hosts and pathogens (4). Pathogen recognition by C-type lectins triggers signaling pathways that lead to the expression of specific cytokines which subsequently instruct adaptive T helper immune responses (4).

1. Hoffmann, S. et al. (2007) J. Biol. Chem. 282:22370.
2. Huysamen, C. et al. (2009) FEMS Microbiol. Lett. 290:121.
3. Monteiro, J.T. and B. Lepenies (2017) Viruses 9:59.
4. van den Berg, L.M. et al. (2012) Ann N Y Acad Sci. 1253:149.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q2HXU8()