CLEC-1 Antibody (219003)

• Reactivity: Hu
• Applications: WB
• Clone: 219003
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CLEC-1 Antibody (219003) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CLEC-1
  Gln77-Asp280
  Accession # Q8NC01
• Specificity: Detects human CLEC-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CLEC-2 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLEC1A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC-1 Antibody (219003)

• CLEC1
• CLEC-1
• CLEC1A
• CLEC1C-type lectin domain family 1 member A
• C-type lectin domain family 1, member A
• C-type lectin-like receptor 1
• C-type lectin-like receptor-1
• MGC34328

Background

CLEC-1 (C-type lectin-like receptor-1) is a type 2 transmembrane protein that belongs to the Dectin-1 family of C-type lectins (1, 2). It consists of a 52 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 207 aa extracellular domain (ECD) (3). The ECD contains a carbohydrate recognition domain (CRD) that includes cysteine residues with conserved spacing (4). In other C-type lectins, these cysteines form intrachain disulfide bonds that stabilize the CRD protein fold (4). CLEC-1 is a nonclassical C-type lectin in that its CRD lacks consensus amino acids which mediate calcium dependent carbohydrate binding in other C-type lectins (2, 3). The cytoplasmic domain contains one tyrosine, multiple serine and threonine, and a cluster of aspartic acid residues. The CLEC-1, CLEC-2, Dectin-1, and LOX-1 genes are located within the NK gene complex that also encodes several receptors involved in regulating NK cell activation (3, 5). CLEC-1 is most highly expressed in activated dendritic cells and at lower levels in endothelial cells and monocytes (3, 5). Within the CRD, human CLEC‑1 shares 65% aa sequence identity with mouse and rat CLEC-1 and 26%‑36% with the other human Dectin-1 family lectins CLEC-2, CLEC9A, CLEC12B, Dectin-1, LOX-1, and MICL.

1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
2. Pyz, E. et al. (2006) Ann. Med. 38:242.
3. Colonna, M. et al. (2000) Eur. J. Immunol. 30:697.
4. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
5. Sobanov, Y. et al. (2001) Eur. J. Immunol. 31:3493.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CLEC-1 Antibody (MAB1704)(3)

Publications using MAB1704

• M Drouin, J Saenz, V Gauttier, B Evrard, G Teppaz, S Pengam, C Mary, A Desselle, V Thepenier, E Wilhelm, E Merieau, C Ligeron, I Girault, MD Lopez, C Fourgeux, D Sinha, I Baccelli, A Moreau, C Louvet, R Josien, J Poschmann, N Poirier, E Chiffoleau CLEC-1 is a death sensor that limits antigen cross-presentation by dendritic cells and represents a target for cancer immunotherapy Science Advances, 2022-11-18;8(46):eabo7621. 2022-11-18 [PMID: 36399563] (Western Blot, Human)
• Zhu G, Lyu L, Yang H et al. CLEC-1 Acts as a Negative Regulator of Dectin-1 Induced Host Inflammatory Response Signature in Aspergillus fumigatus Keratitis Investigative ophthalmology & visual science 5/3/2021 [PMID: 34043748]
• Yohei Takahashi, Hidenori Wake, Masakiyo Sakaguchi, Yukinori Yoshii, Kiyoshi Teshigawara, Dengli Wang et al. Histidine-Rich Glycoprotein Stimulates Human Neutrophil Phagocytosis and Prolongs Survival through CLEC1A The Journal of Immunology 2021-02-15 [PMID: 33452125] (Neutralization, Human)

Bioinformatics

• Gene Symbol: CLEC1A
• Uniprot:
  • Q8NC01()