Claudin-17 Antibody (410509) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with human Claudin-17 was stained with Mouse Anti-Human Claudin-17 Monoclonal Antibody (Catalog # MAB4619, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Claudin-17 Monoclonal Antibody (Catalog # MAB4619, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, Flow
• Clone: 410509
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Claudin-17 Antibody (410509) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human Claudin-17
  Met1-Val224
  Accession # P56750
• Specificity: Detects human Claudin‑17. Stains human Claudin-17 transfectants but not irrelevant transfectants in flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLDN17
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Claudin-17 Antibody (410509) [Unconjugated]

• claudin 17
• Claudin17
• Claudin-17
• CLDN17
• human CLDN17 gene for claudin-17, 10claudin-17
• MGC126552
• MGC126554

Background

Claudin-17 is a 224 amino acid multipass membrane protein in the Claudin family of epithelial tight junction proteins. Human Claudin-17 shares 80%‑82% aa sequence identity with mouse and rat Claudin-17.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Claudin-17 Antibody (MAB4619)(1)

Publication using MAB4619

• S Izraely, S Ben-Menach, O Sagi-Assif, T Meshel, DM Marzese, S Ohe, I Zubrilov, M Pasmanik-C, DSB Hoon, IP Witz ANGPTL4 promotes the progression of cutaneous melanoma to brain metastasis Oncotarget, 2017-07-05;8(44):75778-75796. 2017-07-05 [PMID: 29100268] (Flow Cytometry, Human)

Review for Claudin-17 Antibody (MAB4619) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IF Human 02/20/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 22402829
• Species: Human

Bioinformatics

• Gene Symbol: CLDN17
• Uniprot:
  • P56750()