CIZ1 Antibody

Western Blot: CIZ1 Antibody [NBP2-26207] - Staining of HeLa nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CIZ1 Antibody Summary

• Immunogen: Peptide with sequence C-QPNTQDKTPSKVTAR corresponding to C-Terminus according to NP_036259.2, NP_001124487.1, NP_001124489.1, NP_001124490.1, NP_001244904.1, NP_001244905.1.
• Specificity: This antibody is expected to recognize all reported isoforms (NP_036259.2; NP_001124487.1; NP_001124489.1; NP_001124490.1; NP_001244904.1; NP_001244905.1). Reported variants represent identical protein: NP_036259.2, NP_001124488.1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CIZ1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 90 kDa band observed in HeLa nuclear lysates (calculated MW of 91.2 kDa band according to NP_001124490.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CIZ1 Antibody

• CDKN1A interacting zinc finger protein 1
• CDKN1A-interacting zinc finger protein 1
• cip1-interacting zinc finger protein
• LSFR1ZNF356Nuclear protein NP94
• NP94
• Zinc finger protein 356

Background

CIZ1 (Cip1 interacting zinc-finger protein) was identified as a protein that associates with the cell-cycle inhibiting protein, p21 (Cip1/Waf1). CIZ1 is a member of the matrin 3 protein family and has been shown to bind DNA consensus sequences and co-localize with PCNA during replication. In breast cancer cells, CIZ1 was shown to associate with Cdk2 and dynein light chain 1 (DLC1), a cytoskeletal signaling protein involved in cell cycle regulation and tumorigenesis. Studies of the role of CIZ1 in breast tumorigenesis revealed that CIZ1 is an estrogen-responsive gene that functions as a coactivator of the estrogen receptor. Targeted depletion of CIZ1 inhibits entry into S-phase. This finding along with evidence that CIZ1 interacts with multiple cell cycle regulatory proteins and localizes to DNA replication foci, indicates that CIZ1 plays a critical role in the regulation of DNA replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CIZ1 Antibody (NBP2-26207)(1)

Publication using NBP2-26207

• Xiao J, Uitti RJ, Zhao Y et al. Mutations in CIZ1 cause adult onset primary cervical dystonia. Ann Neurol 2012-04-01 [PMID: 22447717]

Bioinformatics

• Gene Symbol: CIZ1
• Entrez:
  • 25792(Human)